Summary of 9p21 LOH and Immunohistochemical Analyses of p16 and p53 in Progressed FCL
| . | Case No. . | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | ||||||||||||
| Histology | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG |
| p16 PCR | +/+ | −/− | +/+ | +/− | +/+ | −/− | +/+ | +/− | +/+ | −/− | +/+ | +/+ | +/+ | −/− | +/+ | +/+ | +/+ | −/− | +/+ | +/+ | +/+ | −/− |
| p16 IHC | 3+ | − | 3+ | 3+ | TI | TI | 3+ | 3+ | 3+ | − | 3+ | 3+ | 3+ | − | 3+ | 3+ | 3+ | −/+ | 3+ | 3+ | TI | TI |
| p53 IHC | + | + | − | − | − | − | 2+ | 3+ | − | + | + | − | − | + | − | + | − | + | − | − | − | 2+ |
| . | Case No. . | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | ||||||||||||
| Histology | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG | LG | HG |
| p16 PCR | +/+ | −/− | +/+ | +/− | +/+ | −/− | +/+ | +/− | +/+ | −/− | +/+ | +/+ | +/+ | −/− | +/+ | +/+ | +/+ | −/− | +/+ | +/+ | +/+ | −/− |
| p16 IHC | 3+ | − | 3+ | 3+ | TI | TI | 3+ | 3+ | 3+ | − | 3+ | 3+ | 3+ | − | 3+ | 3+ | 3+ | −/+ | 3+ | 3+ | TI | TI |
| p53 IHC | + | + | − | − | − | − | 2+ | 3+ | − | + | + | − | − | + | − | + | − | + | − | − | − | 2+ |
Abbreviations: LOH, loss of heterozygosity; LG, low-grade follicle center lymphoma; HG, high-grade follicle center lymphoma; PCR, polymerase chain reaction, (+/+) retention of both alleles, (+/−) hemizygous deletion, (−/−) homozygous deletion; IHC, immunohistochemistry, (−) <10%, (−/+) 10% to 25%, (+) 26% to 50%, (2+) 51% to 75%, (3+) >75% showed nuclear reactivity with the antibody; TI, technically inadequate.