Effect of Late Withdrawal of IFN-α on Antiviral Activity in IFN-Sensitive (DWS) Daudi Cells Infected With Measles Virus
| Experiment . | Supernatant Virus* . | Cell-Associated Virus* . | |||
|---|---|---|---|---|---|
| IFN-α . | Washed . | Titer . | Inhibition† . | Titer . | Inhibition† . |
| − | − | 5.0 | 4.2 | ||
| +‡ | − | 3.5 | 97% | 3.0 | 94% |
| + | +2-153 | 3.8 | 94% | 3.7 | 68% |
| Experiment . | Supernatant Virus* . | Cell-Associated Virus* . | |||
|---|---|---|---|---|---|
| IFN-α . | Washed . | Titer . | Inhibition† . | Titer . | Inhibition† . |
| − | − | 5.0 | 4.2 | ||
| +‡ | − | 3.5 | 97% | 3.0 | 94% |
| + | +2-153 | 3.8 | 94% | 3.7 | 68% |
Cells infected with measles virus (1 TCID50/cell).
Log10 virus titers (TCID50 per milliliter) of virus released at 48 hours. Culture fluids were microtitrated on VERO monolayers for 6 days.
Inhibition of viral yield, as compared with the controls without IFN-α.
Cells were incubated with IFN-α (100 U/mL) for 18 hours before and throughout virus infection.
After treatment with IFN-α for 18 hours, cells were washed, infected, and maintained in fresh medium without IFN-α.