Bone Marrow Progenitor Cell Analysis ofmpl−/− IL-3−/− Mutant Mice
| Genotype . | Progenitor Cells per 2.5 × 104 Bone Marrow Cells . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Total . | Granulocyte . | GM . | Macrophage . | Eosinophil . | Megakaryocyte . | Meg/E . | Erythroid . | Blast . | |
| Wild-type | 90 ± 40 | 26 ± 2 | 16 ± 11 | 21 ± 14 | 1 ± 1 | 8 ± 5 | 3 ± 2 | 4 ± 6 | 11 ± 4 |
| mpl+/+ IL-3−/− | 79 ± 20 | 23 ± 5 | 13 ± 1 | 13 ± 4 | 1 ± 2 | 6 ± 2 | 5 ± 2 | 7 ± 8 | 11 ± 5 |
| mpl−/− IL-3+/+ | 35 ± 13 | 8 ± 3 | 8 ± 8 | 9 ± 1 | 2 ± 2 | 3 ± 2 | 3 ± 2 | 1 ± 1 | 1 ± 1 |
| mpl−/− IL-3−/− | 27 ± 8 | 8 ± 5 | 7 ± 4 | 6 ± 4 | 1 ± 1 | 2 ± 2 | 2 ± 1 | 1 ± 1 | 0 |
| Genotype . | Progenitor Cells per 2.5 × 104 Bone Marrow Cells . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Total . | Granulocyte . | GM . | Macrophage . | Eosinophil . | Megakaryocyte . | Meg/E . | Erythroid . | Blast . | |
| Wild-type | 90 ± 40 | 26 ± 2 | 16 ± 11 | 21 ± 14 | 1 ± 1 | 8 ± 5 | 3 ± 2 | 4 ± 6 | 11 ± 4 |
| mpl+/+ IL-3−/− | 79 ± 20 | 23 ± 5 | 13 ± 1 | 13 ± 4 | 1 ± 2 | 6 ± 2 | 5 ± 2 | 7 ± 8 | 11 ± 5 |
| mpl−/− IL-3+/+ | 35 ± 13 | 8 ± 3 | 8 ± 8 | 9 ± 1 | 2 ± 2 | 3 ± 2 | 3 ± 2 | 1 ± 1 | 1 ± 1 |
| mpl−/− IL-3−/− | 27 ± 8 | 8 ± 5 | 7 ± 4 | 6 ± 4 | 1 ± 1 | 2 ± 2 | 2 ± 1 | 1 ± 1 | 0 |
Mean ± standard deviations of colony numbers in replicate cultures stimulated with the combination of SCF, IL-3, and EPO from 3 mice per genotype. Cultures were incubated in 5% CO2 in air for 7 days. Colony numbers and composition were determined by examination of stained cultures at 200× magnification. There were no significant differences for comparison of data frommpl+/+ IL-3−/− mice with wild-type mice, or for comparison of data frommpl−/− IL-3−/− mice withmpl−/− IL-3+/+ mice (P > .1).
Abbreviations: GM, mixed granulocyte/macrophage; Meg/E, mixed megakaryocyte/erythroid.