Hematologic Profile of mpl−/−IL-3−/− Mutant Mice
| . | Genotype . | |||
|---|---|---|---|---|
| Wild-Type . | mpl+/+IL-3−/− . | mpl−/−IL-3+/+ . | mpl−/−IL-3−/− . | |
| Peripheral blood | ||||
| Platelets (×10−6/mL) | 792 ± 80 | 769 ± 74 | 76 ± 39 | 70 ± 25 |
| Hematocrit (%) | 49 ± 4 | 47 ± 1 | 52 ± 2 | 47 ± 3 |
| White cell count (×10−6/mL) | 5.8 ± 2.9 | 3.8 ± 2.0 | 4.6 ± 0.6 | 4.6 ± 2.4 |
| Neutrophils (%) | 9 ± 6 | 13 ± 3 | 9 ± 2 | 10 ± 5 |
| Lymphocytes (%) | 82 ± 7 | 83 ± 4 | 85 ± 3 | 85 ± 5 |
| Monocytes (%) | 6 ± 2 | 3 ± 1 | 5 ± 2 | 4 ± 1 |
| Eosinophils (%) | 3 ± 2 | 1 ± 1 | 1 ± 1 | 1 ± 1 |
| Bone marrow | ||||
| Cellularity (×10−6/femur) | 15.6 ± 5.4 | 15.0 ± 1.4 | 23.0 ± 3.0 | 13.4 ± 5.25-151 |
| Blasts (%) | 3 ± 2 | 3 ± 1 | 2 ± 1 | 2 ± 2 |
| Promyelocytes/myelocytes (%) | 7 ± 4 | 6 ± 1 | 6 ± 2 | 6 ± 2 |
| Metamyelocytes/neutrophils (%) | 28 ± 5 | 30 ± 4 | 21 ± 3 | 23 ± 10 |
| Lymphocytes (%) | 26 ± 8 | 33 ± 4 | 35 ± 4 | 30 ± 13 |
| Monocytes (%) | 10 ± 2 | 4 ± 25-151 | 3 ± 2 | 3 ± 2 |
| Eosinophils (%) | 5 ± 2 | 1 ± 1 | 4 ± 2 | 1 ± 1 |
| Nucleated erythroid cells (%) | 21 ± 9 | 23 ± 4 | 29 ± 3 | 35 ± 17 |
| Megakaryocytes (per 10 fields)5-150 | 66 ± 17 | 83 ± 5 | 8 ± 3 | 7 ± 2 |
| Spleen | ||||
| Weight (mg) | 98 ± 17 | 83 ± 5 | 73 ± 13 | 67 ± 3 |
| Cellularity (×10−6) | 77 ± 20 | 79 ± 17 | 70 ± 20 | 65 ± 15 |
| Blasts (%) | 2 ± 1 | 2 ± 0 | 3 ± 1 | 1 ± 2 |
| Promyelocytes/myelocytes (%) | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 1 |
| Metamyelocytes/neutrophils (%) | 2 ± 1 | 1 ± 2 | 2 ± 2 | 3 ± 2 |
| Lymphocytes (%) | 80 ± 19 | 87 ± 2 | 87 ± 8 | 80 ± 15 |
| Monocytes (%) | 1 ± 1 | 3 ± 15-151 | 1 ± 1 | 1 ± 1 |
| Eosinophils (%) | 0 ± 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Nucleated erythroid cells (%) | 15 ± 19 | 7 ± 0 | 7 ± 0 | 15 ± 11 |
| Megakaryocytes (per 10 fields)5-150 | 9 ± 5 | 11 ± 3 | 0.7 ± 0.6 | 0.7 ± 0.6 |
| Peritoneal cavity | ||||
| Cellularity (×10−6) | 5.4 ± 1.9 | 4.1 ± 0.5 | 7.4 ± 3.4 | 4.6 ± 1.1 |
| Neutrophils (%) | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 1 |
| Lymphocytes (%) | 45 ± 13 | 39 ± 16 | 42 ± 4 | 43 ± 13 |
| Monocytes (%) | 52 ± 12 | 57 ± 14 | 55 ± 3 | 56 ± 13 |
| Eosinophils (%) | 1 ± 1 | 1 ± 1 | 1 ± 2 | 0 ± 1 |
| Mast cells (%) | 2 ± 3 | 3 ± 2 | 2 ± 1 | 1 ± 1 |
| . | Genotype . | |||
|---|---|---|---|---|
| Wild-Type . | mpl+/+IL-3−/− . | mpl−/−IL-3+/+ . | mpl−/−IL-3−/− . | |
| Peripheral blood | ||||
| Platelets (×10−6/mL) | 792 ± 80 | 769 ± 74 | 76 ± 39 | 70 ± 25 |
| Hematocrit (%) | 49 ± 4 | 47 ± 1 | 52 ± 2 | 47 ± 3 |
| White cell count (×10−6/mL) | 5.8 ± 2.9 | 3.8 ± 2.0 | 4.6 ± 0.6 | 4.6 ± 2.4 |
| Neutrophils (%) | 9 ± 6 | 13 ± 3 | 9 ± 2 | 10 ± 5 |
| Lymphocytes (%) | 82 ± 7 | 83 ± 4 | 85 ± 3 | 85 ± 5 |
| Monocytes (%) | 6 ± 2 | 3 ± 1 | 5 ± 2 | 4 ± 1 |
| Eosinophils (%) | 3 ± 2 | 1 ± 1 | 1 ± 1 | 1 ± 1 |
| Bone marrow | ||||
| Cellularity (×10−6/femur) | 15.6 ± 5.4 | 15.0 ± 1.4 | 23.0 ± 3.0 | 13.4 ± 5.25-151 |
| Blasts (%) | 3 ± 2 | 3 ± 1 | 2 ± 1 | 2 ± 2 |
| Promyelocytes/myelocytes (%) | 7 ± 4 | 6 ± 1 | 6 ± 2 | 6 ± 2 |
| Metamyelocytes/neutrophils (%) | 28 ± 5 | 30 ± 4 | 21 ± 3 | 23 ± 10 |
| Lymphocytes (%) | 26 ± 8 | 33 ± 4 | 35 ± 4 | 30 ± 13 |
| Monocytes (%) | 10 ± 2 | 4 ± 25-151 | 3 ± 2 | 3 ± 2 |
| Eosinophils (%) | 5 ± 2 | 1 ± 1 | 4 ± 2 | 1 ± 1 |
| Nucleated erythroid cells (%) | 21 ± 9 | 23 ± 4 | 29 ± 3 | 35 ± 17 |
| Megakaryocytes (per 10 fields)5-150 | 66 ± 17 | 83 ± 5 | 8 ± 3 | 7 ± 2 |
| Spleen | ||||
| Weight (mg) | 98 ± 17 | 83 ± 5 | 73 ± 13 | 67 ± 3 |
| Cellularity (×10−6) | 77 ± 20 | 79 ± 17 | 70 ± 20 | 65 ± 15 |
| Blasts (%) | 2 ± 1 | 2 ± 0 | 3 ± 1 | 1 ± 2 |
| Promyelocytes/myelocytes (%) | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 1 |
| Metamyelocytes/neutrophils (%) | 2 ± 1 | 1 ± 2 | 2 ± 2 | 3 ± 2 |
| Lymphocytes (%) | 80 ± 19 | 87 ± 2 | 87 ± 8 | 80 ± 15 |
| Monocytes (%) | 1 ± 1 | 3 ± 15-151 | 1 ± 1 | 1 ± 1 |
| Eosinophils (%) | 0 ± 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| Nucleated erythroid cells (%) | 15 ± 19 | 7 ± 0 | 7 ± 0 | 15 ± 11 |
| Megakaryocytes (per 10 fields)5-150 | 9 ± 5 | 11 ± 3 | 0.7 ± 0.6 | 0.7 ± 0.6 |
| Peritoneal cavity | ||||
| Cellularity (×10−6) | 5.4 ± 1.9 | 4.1 ± 0.5 | 7.4 ± 3.4 | 4.6 ± 1.1 |
| Neutrophils (%) | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 1 |
| Lymphocytes (%) | 45 ± 13 | 39 ± 16 | 42 ± 4 | 43 ± 13 |
| Monocytes (%) | 52 ± 12 | 57 ± 14 | 55 ± 3 | 56 ± 13 |
| Eosinophils (%) | 1 ± 1 | 1 ± 1 | 1 ± 2 | 0 ± 1 |
| Mast cells (%) | 2 ± 3 | 3 ± 2 | 2 ± 1 | 1 ± 1 |
Mean ± standard deviations of data from 3 to 6 mice of each genotype.
Enumerated from histological sections at 200× (spleen) or 400× (bone marrow) magnification, with a minimum of 20 fields counted per mouse.
P < .05 for pairwise comparison of data frommpl+/+ IL-3−/− with wild-type mice or for comparison of data from mpl−/−IL-3−/− mice with mpl−/−IL-3+/+ mice. No other significant differences existed (P > .05).