Platelet Counts and In Vitro Hematopoietic Colony Formation in Mice Defective for Both c-Mpl and IL-3Rα
| Genotype . | No. . | Platelet Count3-150 (×109/L) . | Colony Formation per 50,000 BM Cells3-151 . | ||
|---|---|---|---|---|---|
| Culture Stimulus . | |||||
| IL-3 . | GM-CSF . | Saline . | |||
| NZB (Il3ran/n) | 5 | 870 ± 149 | 2.5 ± 1.53-152 | 48 ± 10 | 0.5 ± 0.5 |
| mpl−/− | 6 | 56 ± 17 | ND | ND | ND |
| F1 (mpl+/−Il3ra+/n) | 16 | 1,060 ± 163 | ND | ND | ND |
| F2mpl−/−Il3ran/n | 6 | 71 ± 43 | 3.5 ± 2.53-152 | 30 ± 3 | 0.5 ± 0.5 |
| mpl−/−Il3ra+/(+ or n) | 20 | 69 ± 33 | 26 ± 7 | 28 ± 5 | 0 ± 0 |
| Genotype . | No. . | Platelet Count3-150 (×109/L) . | Colony Formation per 50,000 BM Cells3-151 . | ||
|---|---|---|---|---|---|
| Culture Stimulus . | |||||
| IL-3 . | GM-CSF . | Saline . | |||
| NZB (Il3ran/n) | 5 | 870 ± 149 | 2.5 ± 1.53-152 | 48 ± 10 | 0.5 ± 0.5 |
| mpl−/− | 6 | 56 ± 17 | ND | ND | ND |
| F1 (mpl+/−Il3ra+/n) | 16 | 1,060 ± 163 | ND | ND | ND |
| F2mpl−/−Il3ran/n | 6 | 71 ± 43 | 3.5 ± 2.53-152 | 30 ± 3 | 0.5 ± 0.5 |
| mpl−/−Il3ra+/(+ or n) | 20 | 69 ± 33 | 26 ± 7 | 28 ± 5 | 0 ± 0 |
Abbreviation: ND, not determined.
Mean ± standard deviation of data for the number of mice indicated for each genotype. Mice were analyzed at 6 to 8 weeks of age for F1 and F2 mice and at 3 to 5 months of age for NZB andmpl−/− mice.
Mean ± standard deviation of data from colony assays containing no stimulus (saline), IL-3, or GM-CSF and maintained in a fully humidified atmosphere of 5% CO2 in air for 7 days.
P < .01 for comparison with GM-CSF–stimulated cultures from the same mice.