Cytokine Production in mpl−/−Mice
| Organ . | IL-3 (ng) . | LIF (ng) . | IL-6 (ng) . | IL-11 (ng) . | GM-CSF (ng) . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | |
| Salivary gland | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 ± 4 | 12 ± 14 |
| Thymus | 0 | 0 | 3 ± 0 | 8 ± 4 | 28 ± 15 | 83 ± 61 | 2 ± 1 | 2 ± 1 | 24 ± 11 | 24 ± 11 |
| Lung | 0 | 0 | 50 ± 42 | 100 ± 85 | 660 ± 877 | 800 ± 679 | 8 ± 4 | 10 ± 0 | 1,312 ± 925 | 1,792 ± 512 |
| Heart | 0 | 0 | 4 ± 2 | 6 ± 5 | 18 ± 17 | 55 ± 70 | 0 | 0 | 48 ± 23 | 64 ± 0 |
| Liver | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Spleen | 0 | 0 | 5 ± 7 | 10 ± 0 | 163 ± 223 | 100 ± 85 | 0 | 0 | 6 ± 3 | 5 ± 4 |
| Kidney | 0 | 0 | 2 ± 1 | 3 ± 4 | 10 ± 14 | 4 ± 2 | 0 | 0 | 40 ± 34 | 36 ± 40 |
| Muscle | 0 | 0 | 47 ± 40 | 94 ± 80 | 305 ± 417 | 375 ± 225 | 0 | 0 | 105 ± 99 | 338 ± 424 |
| Bone marrow | 0 | 0 | 4 ± 1 | 6 ± 3 | 8 ± 7 | 61 ± 84 | 0 | 0 | 0 | 0 |
| Bone shaft | 0 | 0 | 19 ± 16 | 30 ± 0 | 248 ± 329 | 300 ± 225 | 13 ± 4 | 20 ± 14 | 0 | 0 |
| Bladder | 0 | 0 | 8 ± 4 | 10 ± 0 | 85 ± 106 | 80 ± 0 | 10 ± 0 | 10 ± 0 | 30 ± 25 | 48 ± 18 |
| Brain | 0 | 0 | 1 ± 1 | 1 ± 2 | 6 ± 5 | 8 ± 4 | 0 | 0 | 1 ± 1 | 1 ± 1 |
| Testes | 0 | 0 | 4 ± 2 | 5 ± 0 | 11 ± 12 | 6 ± 5 | 0 | 0 | 12 ± 6 | 12 ± 6 |
| Serum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Detection limit (pg) | 20 | 200 | 100 | 100 | 100 | |||||
| Organ . | IL-3 (ng) . | LIF (ng) . | IL-6 (ng) . | IL-11 (ng) . | GM-CSF (ng) . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | mpl+/+ . | mpl−/− . | |
| Salivary gland | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 ± 4 | 12 ± 14 |
| Thymus | 0 | 0 | 3 ± 0 | 8 ± 4 | 28 ± 15 | 83 ± 61 | 2 ± 1 | 2 ± 1 | 24 ± 11 | 24 ± 11 |
| Lung | 0 | 0 | 50 ± 42 | 100 ± 85 | 660 ± 877 | 800 ± 679 | 8 ± 4 | 10 ± 0 | 1,312 ± 925 | 1,792 ± 512 |
| Heart | 0 | 0 | 4 ± 2 | 6 ± 5 | 18 ± 17 | 55 ± 70 | 0 | 0 | 48 ± 23 | 64 ± 0 |
| Liver | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Spleen | 0 | 0 | 5 ± 7 | 10 ± 0 | 163 ± 223 | 100 ± 85 | 0 | 0 | 6 ± 3 | 5 ± 4 |
| Kidney | 0 | 0 | 2 ± 1 | 3 ± 4 | 10 ± 14 | 4 ± 2 | 0 | 0 | 40 ± 34 | 36 ± 40 |
| Muscle | 0 | 0 | 47 ± 40 | 94 ± 80 | 305 ± 417 | 375 ± 225 | 0 | 0 | 105 ± 99 | 338 ± 424 |
| Bone marrow | 0 | 0 | 4 ± 1 | 6 ± 3 | 8 ± 7 | 61 ± 84 | 0 | 0 | 0 | 0 |
| Bone shaft | 0 | 0 | 19 ± 16 | 30 ± 0 | 248 ± 329 | 300 ± 225 | 13 ± 4 | 20 ± 14 | 0 | 0 |
| Bladder | 0 | 0 | 8 ± 4 | 10 ± 0 | 85 ± 106 | 80 ± 0 | 10 ± 0 | 10 ± 0 | 30 ± 25 | 48 ± 18 |
| Brain | 0 | 0 | 1 ± 1 | 1 ± 2 | 6 ± 5 | 8 ± 4 | 0 | 0 | 1 ± 1 | 1 ± 1 |
| Testes | 0 | 0 | 4 ± 2 | 5 ± 0 | 11 ± 12 | 6 ± 5 | 0 | 0 | 12 ± 6 | 12 ± 6 |
| Serum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Detection limit (pg) | 20 | 200 | 100 | 100 | 100 | |||||
Mean ± standard deviation of the calculated total amount of each cytokine produced in medium conditioned by the entire organ over 4 days. Results from 2 to 4 mice for each assay are shown. Toxicity of media conditioned by liver, kidney, salivary gland, and brain reduced the detection limit for those samples by eightfold.