Yields of DC Cultured in the Presence or Absence of TNFα
| Experiment No. . | TNFα Treatment (×105)* . | |
|---|---|---|
| − . | +-151 . | |
| 1 | 1.0-152 | 2.6 |
| 2 | 1.9 | 2.9 |
| 3 | 1.2 | 2.5 |
| 4 | 1.3 | 3.5 |
| 5 | 1.5 | 2.8 |
| 6 | 1.1 | 2.5 |
| 7 | 1.3 | 2.7 |
| Mean ± SD | 1.3 ± 0.3 | 2.8 ± 0.4 |
| 8 | 1.2 | 3.0 |
| 2.3-153 | ||
| 0.9-155 | ||
| Experiment No. . | TNFα Treatment (×105)* . | |
|---|---|---|
| − . | +-151 . | |
| 1 | 1.0-152 | 2.6 |
| 2 | 1.9 | 2.9 |
| 3 | 1.2 | 2.5 |
| 4 | 1.3 | 3.5 |
| 5 | 1.5 | 2.8 |
| 6 | 1.1 | 2.5 |
| 7 | 1.3 | 2.7 |
| Mean ± SD | 1.3 ± 0.3 | 2.8 ± 0.4 |
| 8 | 1.2 | 3.0 |
| 2.3-153 | ||
| 0.9-155 | ||
*Fourteen-day cultures of DC generated in the presence of human recombinant GM-CSF and IL-4 as described in the Materials and Methods.
Recombinant human TNFα (10 ng/mL) was added to DC cultures at day 7.
Number of DC harvested at day 14 (per 5 × 106 PBMC initially plated).
TNFα was removed from DC cultures after 4 days and the cells were replated in fresh SFM with GM-CSF and IL-4 (without additional TNFα).
TNFα was removed from DC cultures after 2 days and the cells were replated in fresh SFM with GM-CSF and IL-4 (without added TNFα).