Effects of Combinations of 9CTT and Retinoid Analogues on Growth Inhibition, NBT Reduction, and CD11b Expression in NB4 Cells
| Compounds . | Growth (% of control) . | NBT Reduction (A560/107cells) . | CD11b Expression (mean intensity) . | |||
|---|---|---|---|---|---|---|
| −9CTT . | +9CTT . | −9CTT . | +9CTT . | −9CTT . | +9CTT . | |
| None | 100 | 109 ± 4 | 0.9 ± 0.1 | 2.1 ± 0.2 | 1.56 ± 0.03 | 2.26 ± 0.09 |
| Am80 | ||||||
| 3 × 10−10 mol/L | 113 ± 3 | 70 ± 10 | 1.3 ± 0.2 | 9.7 ± 2.3 | 2.21 ± 0.12 | 3.45 ± 0.15 |
| 6 × 10−10 mol/L | 89 ± 4 | 55 ± 3 | 5.6 ± 0.2 | 14.7 ± 1.3 | 3.06 ± 0.12 | 3.66 ± 0.12 |
| Am580 | ||||||
| 1 × 10−10 mol/L | 113 ± 3 | 84 ± 4 | 1.1 ± 0.0 | 8.6 ± 0.9 | 2.09 ± 0.05 | 3.17 ± 0.13 |
| 2 × 10−10 mol/L | 103 ± 8 | 67 ± 3 | 3.4 ± 0.6 | 13.3 ± 0.3 | 2.56 ± 0.21 | 3.39 ± 0.13 |
| Ch55 | ||||||
| 1.5 × 10−10 mol/L | 110 ± 3 | 82 ± 6 | 1.6 ± 0.2 | 7.2 ± 1.7 | 2.55 ± 0.29 | 3.68 ± 0.10 |
| 3 × 10−10 mol/L | 93 ± 1 | 66 ± 4 | 5.7 ± 0.4 | 11.8 ± 2.3 | 3.44 ± 0.41 | 4.36 ± 0.31 |
| Re80 | ||||||
| 1.5 × 10−10 mol/L | 111 ± 2 | 66 ± 7 | 1.4 ± 0.1 | 12.1 ± 0.8 | 2.76 ± 0.24 | 4.00 ± 0.27 |
| 3 × 10−10 mol/L | 85 ± 7 | 51 ± 3 | 5.7 ± 1.5 | 16.7 ± 0.6 | 3.67 ± 0.27 | 4.35 ± 0.17 |
| Am555s | ||||||
| 3 × 10−9 mol/L | 104 ± 4 | 71 ± 5 | 3.9 ± 0.7 | 12.9 ± 0.5 | 3.05 ± 0.15 | 4.30 ± 0.29 |
| 6 × 10−9 mol/L | 83 ± 5 | 56 ± 3 | 10.7 ± 1.0 | 16.5 ± 1.2 | 3.93 ± 0.14 | 4.61 ± 0.07 |
| Compounds . | Growth (% of control) . | NBT Reduction (A560/107cells) . | CD11b Expression (mean intensity) . | |||
|---|---|---|---|---|---|---|
| −9CTT . | +9CTT . | −9CTT . | +9CTT . | −9CTT . | +9CTT . | |
| None | 100 | 109 ± 4 | 0.9 ± 0.1 | 2.1 ± 0.2 | 1.56 ± 0.03 | 2.26 ± 0.09 |
| Am80 | ||||||
| 3 × 10−10 mol/L | 113 ± 3 | 70 ± 10 | 1.3 ± 0.2 | 9.7 ± 2.3 | 2.21 ± 0.12 | 3.45 ± 0.15 |
| 6 × 10−10 mol/L | 89 ± 4 | 55 ± 3 | 5.6 ± 0.2 | 14.7 ± 1.3 | 3.06 ± 0.12 | 3.66 ± 0.12 |
| Am580 | ||||||
| 1 × 10−10 mol/L | 113 ± 3 | 84 ± 4 | 1.1 ± 0.0 | 8.6 ± 0.9 | 2.09 ± 0.05 | 3.17 ± 0.13 |
| 2 × 10−10 mol/L | 103 ± 8 | 67 ± 3 | 3.4 ± 0.6 | 13.3 ± 0.3 | 2.56 ± 0.21 | 3.39 ± 0.13 |
| Ch55 | ||||||
| 1.5 × 10−10 mol/L | 110 ± 3 | 82 ± 6 | 1.6 ± 0.2 | 7.2 ± 1.7 | 2.55 ± 0.29 | 3.68 ± 0.10 |
| 3 × 10−10 mol/L | 93 ± 1 | 66 ± 4 | 5.7 ± 0.4 | 11.8 ± 2.3 | 3.44 ± 0.41 | 4.36 ± 0.31 |
| Re80 | ||||||
| 1.5 × 10−10 mol/L | 111 ± 2 | 66 ± 7 | 1.4 ± 0.1 | 12.1 ± 0.8 | 2.76 ± 0.24 | 4.00 ± 0.27 |
| 3 × 10−10 mol/L | 85 ± 7 | 51 ± 3 | 5.7 ± 1.5 | 16.7 ± 0.6 | 3.67 ± 0.27 | 4.35 ± 0.17 |
| Am555s | ||||||
| 3 × 10−9 mol/L | 104 ± 4 | 71 ± 5 | 3.9 ± 0.7 | 12.9 ± 0.5 | 3.05 ± 0.15 | 4.30 ± 0.29 |
| 6 × 10−9 mol/L | 83 ± 5 | 56 ± 3 | 10.7 ± 1.0 | 16.5 ± 1.2 | 3.93 ± 0.14 | 4.61 ± 0.07 |
Cells (5 × 104 cells/mL) were cultured with retinoid analogues in the absence or presence of 3 × 10−6 mol/L 9CTT for 4 days. Values represent the means ± SD of three separate experiments.