Effects of TT and 9CTT on the Proliferation and Differentiation of Human Myeloid Leukemia Cells
| Cell . | Treatment . | Growth (% of control) . | Cell Cycle (%) . | Morphological Differentiation-150 (%) . | CD11b (mean intensity) . | ||
|---|---|---|---|---|---|---|---|
| G1 . | G2/M . | S . | |||||
| NB4 | None | 100 | 38 ± 2 | 13 ± 1 | 49 ± 1 | 1 ± 0 | 1.7 ± 0.0 |
| TT | 85 ± 8 | 36 ± 1 | 16 ± 1 | 48 ± 1 | 2 ± 1 | 3.8 ± 0.0 | |
| 9CTT | 44 ± 5 | 58 ± 2 | 11 ± 1 | 30 ± 1 | 49 ± 8 | 4.5 ± 0.2 | |
| HT93 | None | 100 | 41 ± 2 | 10 ± 1 | 49 ± 1 | 0 ± 0 | 1.7 ± 0.0 |
| TT | 103 ± 3 | 61 ± 2 | 8 ± 1 | 31 ± 1 | 3 ± 1 | 3.1 ± 0.1 | |
| 9CTT | 36 ± 2 | 72 ± 1 | 10 ± 0 | 18 ± 1 | 54 ± 1 | 4.3 ± 0.1 | |
| U937 | None | 100 | 0 ± 0 | 1.4 ± 0.0 | |||
| TT | 72 ± 3 | ND | 18 ± 3 | 1.8 ± 0.0 | |||
| 9CTT | 41 ± 1 | 30 ± 3 | 2.1 ± 0.1 | ||||
| HL-60 | None | 100 | 0 ± 0 | 2.9 ± 0.3 | |||
| TT | 64 ± 4 | ND | 2 ± 1 | 1.8 ± 0.1 | |||
| 9CTT | 58 ± 6 | 6 ± 0 | 1.9 ± 0.0 | ||||
| Cell . | Treatment . | Growth (% of control) . | Cell Cycle (%) . | Morphological Differentiation-150 (%) . | CD11b (mean intensity) . | ||
|---|---|---|---|---|---|---|---|
| G1 . | G2/M . | S . | |||||
| NB4 | None | 100 | 38 ± 2 | 13 ± 1 | 49 ± 1 | 1 ± 0 | 1.7 ± 0.0 |
| TT | 85 ± 8 | 36 ± 1 | 16 ± 1 | 48 ± 1 | 2 ± 1 | 3.8 ± 0.0 | |
| 9CTT | 44 ± 5 | 58 ± 2 | 11 ± 1 | 30 ± 1 | 49 ± 8 | 4.5 ± 0.2 | |
| HT93 | None | 100 | 41 ± 2 | 10 ± 1 | 49 ± 1 | 0 ± 0 | 1.7 ± 0.0 |
| TT | 103 ± 3 | 61 ± 2 | 8 ± 1 | 31 ± 1 | 3 ± 1 | 3.1 ± 0.1 | |
| 9CTT | 36 ± 2 | 72 ± 1 | 10 ± 0 | 18 ± 1 | 54 ± 1 | 4.3 ± 0.1 | |
| U937 | None | 100 | 0 ± 0 | 1.4 ± 0.0 | |||
| TT | 72 ± 3 | ND | 18 ± 3 | 1.8 ± 0.0 | |||
| 9CTT | 41 ± 1 | 30 ± 3 | 2.1 ± 0.1 | ||||
| HL-60 | None | 100 | 0 ± 0 | 2.9 ± 0.3 | |||
| TT | 64 ± 4 | ND | 2 ± 1 | 1.8 ± 0.1 | |||
| 9CTT | 58 ± 6 | 6 ± 0 | 1.9 ± 0.0 | ||||
Cells (5 × 104 cells were incubated with or without 1.5 × 10−5 mol/L TT or 9CTT for 4 days. Values represent the means ± SD of three separate experiments.
Abbreviation: ND, not done.
Cells differentiating into myelocytes and more mature cells were counted.