Lys C Peptide Number, the Residues in Each Peptide With Each Theoretical Mass, and the Masses Observed for rhAT and phAT by LC/MS
| Peptide . | Residues . | Theoretical . | Observed Mass . | |
|---|---|---|---|---|
| rhAT . | phAT . | |||
| K1 | 1-11 | 1,232.44 | 1,232.3 | 1,232.2 |
| K2 | 12-28 | 2,152.61 | 2,152.5 | 2,152.6 |
| K3 | 29-29 | 146.20 | — | — |
| K4 | 30-39 | 1,093.08 | 1,093.2 | 1,093.0 |
| K5 | 40-53 | 1,698.95 | 1,698.9 | 1,698.9 |
| K6 | 54-70 | 1,957.14 | 1,957.2 | 1,957.2 |
| K7 | 71-91 | 2,299.60 | 2,299.7 | 2,299.7 |
| K8* | 92-107 | 3,976.18 | 3,976.6 | 4,121.9 |
| K9 | 108-114 | 838.92 | 838.7 | 838.7 |
| K10 | 115-125 | 1,340.51 | 1,340.4 | 1,340.5 |
| K11 | 126-133 | 1,170.46 | 1,170.2 | 1,170.3 |
| K12* | 134-136 | 331.38 | — | — |
| K13 | 137-139 | 320.35 | — | — |
| K14 | 140-150 | 1,219.42 | 1,219.2 | 1,219.2 |
| K15* | 151-169 | 3,557.63 | 3,558.2 | 4,385.3 |
| K16 | 170-176 | 860.03 | 859.8 | 859.7 |
| K17 | 177-188 | 1,330.43 | 1,330.3 | 1,330.3 |
| K18* | 189-193 | 2,693.63 | 2,693.6 | 2,839.2 |
| K19 | 194-222 | 3,248.78 | 3,249.1 | 3,249.9 |
| K20 | 223-226 | 502.62 | 502.3 | 502.3 |
| K21 | 227-228 | 233.28 | — | — |
| K22 | 229-236 | 978.08 | 978.0 | 977.9 |
| K23 | 237-241 | 698.83 | 698.7 | 698.4 |
| K24 | 242-257 | 1,799.00 | 1,799.0 | 1,798.9 |
| K25 | 258-275 | 2,209.59 | 2,209.4 | 2,209.5 |
| K26 | 276-287 | 1,314.62 | — | — |
| K27 | 288-290 | 372.43 | — | — |
| K28 | 291-294 | 417.51 | 417.2 | 417.1 |
| K29 | 295-297 | 374.45 | — | — |
| K30 | 298-332 | 4,260.98 | 4,261.5 | 4,261.0 |
| K31 | 333-348 | 1,849.10 | 1,849.2 | 1,848.7 |
| K32 | 349-350 | 233.28 | — | — |
| K33 | 351-370 | 2,202.46 | 2,202.4 | 2,202.5 |
| K34 | 371-403 | 3,448.85 | 3,448.8 | 3,449.1 |
| K35 | 404-432 | 3,421.20 | 3,420.9 | 3,421.3 |
| Peptide . | Residues . | Theoretical . | Observed Mass . | |
|---|---|---|---|---|
| rhAT . | phAT . | |||
| K1 | 1-11 | 1,232.44 | 1,232.3 | 1,232.2 |
| K2 | 12-28 | 2,152.61 | 2,152.5 | 2,152.6 |
| K3 | 29-29 | 146.20 | — | — |
| K4 | 30-39 | 1,093.08 | 1,093.2 | 1,093.0 |
| K5 | 40-53 | 1,698.95 | 1,698.9 | 1,698.9 |
| K6 | 54-70 | 1,957.14 | 1,957.2 | 1,957.2 |
| K7 | 71-91 | 2,299.60 | 2,299.7 | 2,299.7 |
| K8* | 92-107 | 3,976.18 | 3,976.6 | 4,121.9 |
| K9 | 108-114 | 838.92 | 838.7 | 838.7 |
| K10 | 115-125 | 1,340.51 | 1,340.4 | 1,340.5 |
| K11 | 126-133 | 1,170.46 | 1,170.2 | 1,170.3 |
| K12* | 134-136 | 331.38 | — | — |
| K13 | 137-139 | 320.35 | — | — |
| K14 | 140-150 | 1,219.42 | 1,219.2 | 1,219.2 |
| K15* | 151-169 | 3,557.63 | 3,558.2 | 4,385.3 |
| K16 | 170-176 | 860.03 | 859.8 | 859.7 |
| K17 | 177-188 | 1,330.43 | 1,330.3 | 1,330.3 |
| K18* | 189-193 | 2,693.63 | 2,693.6 | 2,839.2 |
| K19 | 194-222 | 3,248.78 | 3,249.1 | 3,249.9 |
| K20 | 223-226 | 502.62 | 502.3 | 502.3 |
| K21 | 227-228 | 233.28 | — | — |
| K22 | 229-236 | 978.08 | 978.0 | 977.9 |
| K23 | 237-241 | 698.83 | 698.7 | 698.4 |
| K24 | 242-257 | 1,799.00 | 1,799.0 | 1,798.9 |
| K25 | 258-275 | 2,209.59 | 2,209.4 | 2,209.5 |
| K26 | 276-287 | 1,314.62 | — | — |
| K27 | 288-290 | 372.43 | — | — |
| K28 | 291-294 | 417.51 | 417.2 | 417.1 |
| K29 | 295-297 | 374.45 | — | — |
| K30 | 298-332 | 4,260.98 | 4,261.5 | 4,261.0 |
| K31 | 333-348 | 1,849.10 | 1,849.2 | 1,848.7 |
| K32 | 349-350 | 233.28 | — | — |
| K33 | 351-370 | 2,202.46 | 2,202.4 | 2,202.5 |
| K34 | 371-403 | 3,448.85 | 3,448.8 | 3,449.1 |
| K35 | 404-432 | 3,421.20 | 3,420.9 | 3,421.3 |
*Glycopeptide.