Compilation of the Percent Recovery for Each Step of Purification and Overall for the Process
| Step . | Total ATIII (g) . | Step Recovery . | Overall Yield . |
|---|---|---|---|
| Diluted milk | 115.0 | — | 100% |
| Heparin eluate | 87.3 | 76% | 76% |
| Ultrafiltration | 86.0 | 98% | 75% |
| DEAE eluate | 84.7 | 98% | 74% |
| Methyl eluate | 67.3 | 80% | 59% |
| UF/Formulation | 61.0 | 91% | 53% |
| Step . | Total ATIII (g) . | Step Recovery . | Overall Yield . |
|---|---|---|---|
| Diluted milk | 115.0 | — | 100% |
| Heparin eluate | 87.3 | 76% | 76% |
| Ultrafiltration | 86.0 | 98% | 75% |
| DEAE eluate | 84.7 | 98% | 74% |
| Methyl eluate | 67.3 | 80% | 59% |
| UF/Formulation | 61.0 | 91% | 53% |
Data represent the average of 3 runs at this scale.