Yeast two-hybrid analysis of dimerization capacity of wild-type BAX versus BAX^G67R and BAX^G108V. AD-fusion constructs are indicated across the top lane: control vector pACTII, BAXΔC19, BAX^G67RΔC18, BAX^G108VΔC18, BCL-2ΔC22, BCL-X_LΔC19. DB-fusion constructs are indicated in the left hand column: BAXΔC18, BAX^G67RΔC18, BAX^G108VΔC18, BCL-X_LΔC19. Protein interactions as measured by X-gal substrate conversion are indicated as “−” for no interaction or “+” for clear positive. Interactions that were enhanced are indicated as “++” or “+++.” Interactions were tested in two to four independent experiments.