Detection of HIV-1 p24-Positive Cells and Cytokine mRNA-Expressing Cells by Combined Immunocytochemistry and In Situ Hybridization
| In Situ Hybridization Detecting Cytokine mRNA . | Immunocytochemistry Detecting HIV-1 p24 . | % Cytokine mRNA Positive Cells* . |
|---|---|---|
| IL-6 | p24-positive | 62.7 ± 4.6 |
| p24-negative | 65.1 ± 3.2 | |
| IL-8 | p24-positive | 81.9 ± 3.8 |
| p24-negative | 83.1 ± 1.3 | |
| TNF-α | p24-positive | 44.9 ± 3.6 |
| p24-negative | 47.3 ± 2.3 |
| In Situ Hybridization Detecting Cytokine mRNA . | Immunocytochemistry Detecting HIV-1 p24 . | % Cytokine mRNA Positive Cells* . |
|---|---|---|
| IL-6 | p24-positive | 62.7 ± 4.6 |
| p24-negative | 65.1 ± 3.2 | |
| IL-8 | p24-positive | 81.9 ± 3.8 |
| p24-negative | 83.1 ± 1.3 | |
| TNF-α | p24-positive | 44.9 ± 3.6 |
| p24-negative | 47.3 ± 2.3 |
If the percentage of positive cells was compared between uninfected and HIV-1–infected cultures of the same donor, no statistical significant difference could be found. With the χ2 test α between 0.26 and 0.83 were determined.
HIV-1 p24-positive cells and cytokine mRNA-expressing cells were detected as described in Materials and Methods after 24 hours stimulation with LPS at day 15 after start of culture. Data presented are the mean percentage ± SD of cytokine mRNA positive cells related to HIV-1 p24-positive cells or HIV-1 p24-negative cells, respectively. Presented are the results of seven experiments with cells of different donors.