Table 2.

Secretion of Proinflammatory Cytokines and Hematopoietic Growth Factors by Uninfected and HIV-1–Infected Macrophages

Donor*pg/105Cells†
Uninfected HIV-1– Infected
IL-1β 
 Unstimulated  I  0  0  
 II  0  0  
 III 0  0  
 4 h LPS  I  3.2 ± 0.9  7.8 ± 0.5 
 II  2.7 ± 1.0  8.1 ± 1.1  
 III 3.6 ± 0.8  9.8 ± 2.1  
 24 h LPS  I 10.0 ± 1.0  24.6 ± 0.7  
 II  10.5 ± 0.8 24.8 ± 1.8  
 III  18.7 ± 1.5  41.4 ± 3.4 
IL-6  
 Unstimulated  I  0  0 
 II  0  0  
 III  0 0  
 4 h LPS  I  973 ± 17  2,329 ± 239 
 II  1,561 ± 5.4  4,107 ± 185  
 III 1,159 ± 66  4,029 ± 169  
 24 h LPS  I 1,358 ± 33  3,725 ± 255  
 II  2,320 ± 248 4,914 ± 124  
 III  1,913 ± 152  8,542 ± 231 
IL-8  
 Unstimulated  I  446 ± 56  918 ± 48 
 II  965 ± 115  2,777 ± 184  
 III 765 ± 94  2,032 ± 107  
 4 h LPS  I 7,814 ± 501  15,855 ± 905  
 II 21,855 ± 2,272  52,209 ± 2,364  
 III 11,504 ± 527  35,809 ± 2,670  
 24 h LPS  I 21,416 ± 1,909  61,091 ± 1,799  
 II 41,288 ± 2,049  95,834 ± 6,101  
 III 28,661 ± 1,725  109,612 ± 11,201  
TNF-α 
 Unstimulated  I  0  0  
 II 0  0  
 III  0  0 
 4 h LPS  I  973 ± 31  3,845 ± 46  
 II 2,803 ± 161  8,191 ± 1,079  
 III 1,214 ± 106  2,981 ± 127  
 24 h LPS  I 751 ± 52  3,020 ± 71  
 II  2,621 ± 177 6,939 ± 85  
 III  1,600 ± 186  3,994 ± 188 
G-CSF  
 Unstimulated  I  0  0 
 II  0  0  
 III  0 0  
 4 h LPS  I  488 ± 33  195 ± 15 
 II  852 ± 64  194 ± 20  
 III 483 ± 32  164 ± 24  
 24 h LPS  I 2,197 ± 207  748 ± 58  
 II  2,813 ± 234 488 ± 26  
 III  2,511 ± 156  691 ± 49 
GM-CSF  
 Unstimulated  I  0  0 
 II  0  0  
 III  0 0  
 4 h LPS  I  130 ± 10  55 ± 6 
 II  189 ± 14  79 ± 12  
 III 279 ± 26  52 ± 11 
 24 h LPS  I 576 ± 36  210 ± 21  
 II  607 ± 22 199 ± 18  
 III  816 ± 40  382 ± 34 
Donor*pg/105Cells†
Uninfected HIV-1– Infected
IL-1β 
 Unstimulated  I  0  0  
 II  0  0  
 III 0  0  
 4 h LPS  I  3.2 ± 0.9  7.8 ± 0.5 
 II  2.7 ± 1.0  8.1 ± 1.1  
 III 3.6 ± 0.8  9.8 ± 2.1  
 24 h LPS  I 10.0 ± 1.0  24.6 ± 0.7  
 II  10.5 ± 0.8 24.8 ± 1.8  
 III  18.7 ± 1.5  41.4 ± 3.4 
IL-6  
 Unstimulated  I  0  0 
 II  0  0  
 III  0 0  
 4 h LPS  I  973 ± 17  2,329 ± 239 
 II  1,561 ± 5.4  4,107 ± 185  
 III 1,159 ± 66  4,029 ± 169  
 24 h LPS  I 1,358 ± 33  3,725 ± 255  
 II  2,320 ± 248 4,914 ± 124  
 III  1,913 ± 152  8,542 ± 231 
IL-8  
 Unstimulated  I  446 ± 56  918 ± 48 
 II  965 ± 115  2,777 ± 184  
 III 765 ± 94  2,032 ± 107  
 4 h LPS  I 7,814 ± 501  15,855 ± 905  
 II 21,855 ± 2,272  52,209 ± 2,364  
 III 11,504 ± 527  35,809 ± 2,670  
 24 h LPS  I 21,416 ± 1,909  61,091 ± 1,799  
 II 41,288 ± 2,049  95,834 ± 6,101  
 III 28,661 ± 1,725  109,612 ± 11,201  
TNF-α 
 Unstimulated  I  0  0  
 II 0  0  
 III  0  0 
 4 h LPS  I  973 ± 31  3,845 ± 46  
 II 2,803 ± 161  8,191 ± 1,079  
 III 1,214 ± 106  2,981 ± 127  
 24 h LPS  I 751 ± 52  3,020 ± 71  
 II  2,621 ± 177 6,939 ± 85  
 III  1,600 ± 186  3,994 ± 188 
G-CSF  
 Unstimulated  I  0  0 
 II  0  0  
 III  0 0  
 4 h LPS  I  488 ± 33  195 ± 15 
 II  852 ± 64  194 ± 20  
 III 483 ± 32  164 ± 24  
 24 h LPS  I 2,197 ± 207  748 ± 58  
 II  2,813 ± 234 488 ± 26  
 III  2,511 ± 156  691 ± 49 
GM-CSF  
 Unstimulated  I  0  0 
 II  0  0  
 III  0 0  
 4 h LPS  I  130 ± 10  55 ± 6 
 II  189 ± 14  79 ± 12  
 III 279 ± 26  52 ± 11 
 24 h LPS  I 576 ± 36  210 ± 21  
 II  607 ± 22 199 ± 18  
 III  816 ± 40  382 ± 34 

*Presented are the results of three experiments using cells of different donors (I, II, III).

†

Cytokine secretion was determined by ELISA after 4 and 24 hours stimulation with 100 ng/mL LPS at day 8 after start of culture as described in Materials and Methods. Data presented are the means ± SD of each experiment done in triplicate.

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