Immunocytochemical Evaluation of Intracellular Proinflammatory Cytokines and Hematopoietic Growth Factors in Uninfected and HIV-1–Infected Macrophages
| . | % Positive Cells-150 on Day 8-151 . | % Positive Cells-150 on Day 15-152 . | ||
|---|---|---|---|---|
| Uninfected . | HIV-1– Infected . | Uninfected . | HIV-1– Infected . | |
| IL-1β | ||||
| Unstimulated | 0 | 0 | 0 | 0 |
| 4 h LPS | 73.7 ± 8.3 | 76.1 ± 8.3 | 68.0 ± 2.4 | 66.5 ± 2.1 |
| 24 h LPS | 75.2 ± 11.7-153 | 70.6 ± 10.2-155 | 68.5 ± 2.5-160 | 71.8 ± 2.8-164 |
| IL-6 | ||||
| Unstimulated | 0 | 0 | 0 | 0 |
| 4 h LPS | 12.7 ± 2.4 | 12.1 ± 2.3 | 10.4 ± 0.9 | 11.5 ± 1.1 |
| 24 h LPS | 67.8 ± 11.2 | 68.9 ± 11.3 | 64.9 ± 8.3 | 62.2 ± 12.3 |
| IL-8 | ||||
| Unstimulated | 81.5 ± 11.1 | 77.8 ± 8.5 | 68.0 ± 10.3 | 66.2 ± 11.8 |
| 4 h LPS | 84.1 ± 9.6 | 85.2 ± 8.1 | 77.2 ± 14.6 | 76.1 ± 8.4 |
| 24 h LPS | 84.7 ± 11.2¶ | 85.6 ± 8.1# | 74.9 ± 5.9-161 | 80.9 ± 7.3-162 |
| TNF-α | ||||
| Unstimulated | 0 | 0 | 0 | 0 |
| 4 h LPS | 9.2 ± 1.1 | 10.4 ± 3.1 | 9.7 ± 1.1 | 8.6 ± 0.8 |
| 24 h LPS | 51.8 ± 7.4 | 53.0 ± 7.7 | 50.3 ± 7.8 | 50.1 ± 4.9 |
| G-CSF | ||||
| Unstimulated | 89.4 ± 4.2 | 87.9 ± 3.3 | 81.9 ± 9.3 | 81.2 ± 13.4 |
| 4 h LPS | 92.5 ± 5.0 | 91.2 ± 4.2 | 84.5 ± 7.2 | 85.2 ± 9.0 |
| 24 h LPS | 91.3 ± 6.2 | 91.9 ± 4.3 | 86.2 ± 10.4 | 83.2 ± 9.4 |
| GM-CSF | ||||
| Unstimulated | 93.5 ± 4.9 | 90.4 ± 4.3 | 88.2 ± 6.2 | 89.8 ± 5.6 |
| 4 h LPS | 96.7 ± 2.7 | 94.7 ± 2.9 | 91.9 ± 6.9 | 89.9 ± 5.8 |
| 24 h LPS | 97.4 ± 1.6 | 96.5 ± 1.9 | 91.4 ± 6.8 | 91.3 ± 5.9 |
| . | % Positive Cells-150 on Day 8-151 . | % Positive Cells-150 on Day 15-152 . | ||
|---|---|---|---|---|
| Uninfected . | HIV-1– Infected . | Uninfected . | HIV-1– Infected . | |
| IL-1β | ||||
| Unstimulated | 0 | 0 | 0 | 0 |
| 4 h LPS | 73.7 ± 8.3 | 76.1 ± 8.3 | 68.0 ± 2.4 | 66.5 ± 2.1 |
| 24 h LPS | 75.2 ± 11.7-153 | 70.6 ± 10.2-155 | 68.5 ± 2.5-160 | 71.8 ± 2.8-164 |
| IL-6 | ||||
| Unstimulated | 0 | 0 | 0 | 0 |
| 4 h LPS | 12.7 ± 2.4 | 12.1 ± 2.3 | 10.4 ± 0.9 | 11.5 ± 1.1 |
| 24 h LPS | 67.8 ± 11.2 | 68.9 ± 11.3 | 64.9 ± 8.3 | 62.2 ± 12.3 |
| IL-8 | ||||
| Unstimulated | 81.5 ± 11.1 | 77.8 ± 8.5 | 68.0 ± 10.3 | 66.2 ± 11.8 |
| 4 h LPS | 84.1 ± 9.6 | 85.2 ± 8.1 | 77.2 ± 14.6 | 76.1 ± 8.4 |
| 24 h LPS | 84.7 ± 11.2¶ | 85.6 ± 8.1# | 74.9 ± 5.9-161 | 80.9 ± 7.3-162 |
| TNF-α | ||||
| Unstimulated | 0 | 0 | 0 | 0 |
| 4 h LPS | 9.2 ± 1.1 | 10.4 ± 3.1 | 9.7 ± 1.1 | 8.6 ± 0.8 |
| 24 h LPS | 51.8 ± 7.4 | 53.0 ± 7.7 | 50.3 ± 7.8 | 50.1 ± 4.9 |
| G-CSF | ||||
| Unstimulated | 89.4 ± 4.2 | 87.9 ± 3.3 | 81.9 ± 9.3 | 81.2 ± 13.4 |
| 4 h LPS | 92.5 ± 5.0 | 91.2 ± 4.2 | 84.5 ± 7.2 | 85.2 ± 9.0 |
| 24 h LPS | 91.3 ± 6.2 | 91.9 ± 4.3 | 86.2 ± 10.4 | 83.2 ± 9.4 |
| GM-CSF | ||||
| Unstimulated | 93.5 ± 4.9 | 90.4 ± 4.3 | 88.2 ± 6.2 | 89.8 ± 5.6 |
| 4 h LPS | 96.7 ± 2.7 | 94.7 ± 2.9 | 91.9 ± 6.9 | 89.9 ± 5.8 |
| 24 h LPS | 97.4 ± 1.6 | 96.5 ± 1.9 | 91.4 ± 6.8 | 91.3 ± 5.9 |
If the percentage of positive cells was compared between uninfected and HIV-1–infected cultures of the same donor, no statistical significant difference could be found. With the χ2 test α between 0.35 and 0.923 were determined.
Data represented cytokine positive cells by immunocytochemical analysis (mean ± standard deviation [SD] of 3 donors). The experiments were performed as described in Materials and Methods (see HIV infection of MAC).
According to protocol 1.
According to protocol 2.
Symbols indicate percentage of strongly positive cells.
7.8% ± 1.6%.
7.9% ± 1.7%.
¶49.7% ± 4.6%.
#48.3% ± 7.5%.
7.7% ± 2.4%.
7.8% ± 2.1%.
49.4% ± 1.9%.
46.7% ± 5%.