Immunohistochemical Double Labeling With AAA4 on Human Spleen
| Code . | Specificity . | Concentration Used . | Double Labeling . | Reference/Source . |
|---|---|---|---|---|
| RV202 | Vimentin | 1:10 | − | [21] |
| 5B5 | Fibroblasts | 1:20 | − | DAKO |
| HLE-1 | CD45, leukocytes | 1:100 | + | Becton Dickinson |
| HLA-DR | MHC-II | 1:250 | − | Becton Dickinson |
| Leu4 | CD3, T cells | 1:100 | − | Becton Dickinson |
| B4 | CD19, B cells | 1:100 | − | Coulter Clone |
| Mac | Macrophages | 1:500 | − | DAKO |
| L25 | Dendritic cells | 1:500 | − | [22] |
| LeuM3 | CD14, monocytes | 1:100 | − | Becton Dickinson |
| CD24 | Granulocytes | 1:100 | + | Ortho Diagnostics, Raritan, NJ |
| VIM-D5 | CD15, neutrophilic granulocytes | 1:100 | + | W. Knapp, Vienna, Austria |
| Code . | Specificity . | Concentration Used . | Double Labeling . | Reference/Source . |
|---|---|---|---|---|
| RV202 | Vimentin | 1:10 | − | [21] |
| 5B5 | Fibroblasts | 1:20 | − | DAKO |
| HLE-1 | CD45, leukocytes | 1:100 | + | Becton Dickinson |
| HLA-DR | MHC-II | 1:250 | − | Becton Dickinson |
| Leu4 | CD3, T cells | 1:100 | − | Becton Dickinson |
| B4 | CD19, B cells | 1:100 | − | Coulter Clone |
| Mac | Macrophages | 1:500 | − | DAKO |
| L25 | Dendritic cells | 1:500 | − | [22] |
| LeuM3 | CD14, monocytes | 1:100 | − | Becton Dickinson |
| CD24 | Granulocytes | 1:100 | + | Ortho Diagnostics, Raritan, NJ |
| VIM-D5 | CD15, neutrophilic granulocytes | 1:100 | + | W. Knapp, Vienna, Austria |