Purification of E coli CDD
| Step . | Total Protein (mg) . | Total Activity (U × 10−3) . | Specific Activity (U × 10−3/mg) . | Purification (fold) . | Activity Recovery (%) . |
|---|---|---|---|---|---|
| E coli extract | 32.0 | 11,000 | 340 | 1.0 | 100.0 |
| DEAE-Sephacel | 25.0 | 10,000 | 400 | 1.2 | 91.0 |
| Mono Q | 2.7 | 2,800 | 1,040 | 3.1 | 25.5 |
| Mini Q | 0.6 | 750 | 1,250 | 3.7 | 6.8 |
| Step . | Total Protein (mg) . | Total Activity (U × 10−3) . | Specific Activity (U × 10−3/mg) . | Purification (fold) . | Activity Recovery (%) . |
|---|---|---|---|---|---|
| E coli extract | 32.0 | 11,000 | 340 | 1.0 | 100.0 |
| DEAE-Sephacel | 25.0 | 10,000 | 400 | 1.2 | 91.0 |
| Mono Q | 2.7 | 2,800 | 1,040 | 3.1 | 25.5 |
| Mini Q | 0.6 | 750 | 1,250 | 3.7 | 6.8 |