Table 1. Effect of Anti-FasL and/or... | American Society of Hematology

Table 1.

Effect of Anti-FasL and/or Anti-TNFα Antibodies on GVHD-Associated Lymphoid Hypoplasia

Mice	Cell No. (×10⁷)
	H-2K^d+K^b+			H-2K^d−K^b+
	CD4⁺	CD8⁺	B220⁺	CD4⁺	CD8⁺	B220⁺
Normal	2.1 ± 0.11	1.5 ± 0.57	2.8 ± 0.80	—	—	—
GVHD	0.2 ± 0.02	0.2 ± 0.02	0.7 ± 0.06	0.4 ± 0.06	0.3 ± 0.07	1.3 ± 0.60
Anti-FasL	1.2 ± 0.05	1.7 ± 0.06	1.8 ± 0.33	2.2 ± 0.70	2.5 ± 0.84	1.6 ± 0.52
Anti-TNFα	1.2 ± 0.46	1.1 ± 0.29	1.4 ± 0.02	2.4 ± 0.30	2.0 ± 0.30	1.9 ± 0.10
Anti-FasL + anti-TNFα	1.6 ± 0.20	1.9 ± 0.45	2.0 ± 0.65	3.3 ± 0.27	3.3 ± 0.39	2.8 ± 0.90

Mice	Cell No. (×10⁷)
	H-2K^d+K^b+			H-2K^d−K^b+
	CD4⁺	CD8⁺	B220⁺	CD4⁺	CD8⁺	B220⁺
Normal	2.1 ± 0.11	1.5 ± 0.57	2.8 ± 0.80	—	—	—
GVHD	0.2 ± 0.02	0.2 ± 0.02	0.7 ± 0.06	0.4 ± 0.06	0.3 ± 0.07	1.3 ± 0.60
Anti-FasL	1.2 ± 0.05	1.7 ± 0.06	1.8 ± 0.33	2.2 ± 0.70	2.5 ± 0.84	1.6 ± 0.52
Anti-TNFα	1.2 ± 0.46	1.1 ± 0.29	1.4 ± 0.02	2.4 ± 0.30	2.0 ± 0.30	1.9 ± 0.10
Anti-FasL + anti-TNFα	1.6 ± 0.20	1.9 ± 0.45	2.0 ± 0.65	3.3 ± 0.27	3.3 ± 0.39	2.8 ± 0.90

Cell numbers of CD4⁺ T, CD8⁺ T, and B220⁺ B cells of host (H-2K^d+K^b+) or donor (H-2K^d−K^b+) origin in the splenocytes from normal CBF1, GVHD, or anti-FasL antibody-treated and/or anti-TNFα antibody-treated mice on day 21 were calculated from the total numbers of splenocytes recovered, and the percentages of each subpopulation were determined using three-color flow cytometric analysis. Data represent the mean ± SD of 5 mice in each group.

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