Outgrowth Potential of Histopaque Resolved Molt-4 Cell Types
| Fraction . | Doublings/48 hours . | Doublings/96 hours . | ||
|---|---|---|---|---|
| −20 μmol/L Thymidine . | +20 μmol/L Thymidine . | −20 μmol/L Thymidine . | +20 μmol/L Thymidine . | |
| Control | ND | ND | 5.1 ± 0.2 | 5.0 ± 0.3 |
| Live | 0.7 ± 0.1* | 2.0 ± 0.2† | 0.7 ± 0.1 | 3.9 ± 0.1 |
| Bleb | 0 | 0 | 0 | 0 |
| Dead | 0 | 0 | 0 | 0 |
| Fraction . | Doublings/48 hours . | Doublings/96 hours . | ||
|---|---|---|---|---|
| −20 μmol/L Thymidine . | +20 μmol/L Thymidine . | −20 μmol/L Thymidine . | +20 μmol/L Thymidine . | |
| Control | ND | ND | 5.1 ± 0.2 | 5.0 ± 0.3 |
| Live | 0.7 ± 0.1* | 2.0 ± 0.2† | 0.7 ± 0.1 | 3.9 ± 0.1 |
| Bleb | 0 | 0 | 0 | 0 |
| Dead | 0 | 0 | 0 | 0 |
Log-phase Molt-4 cells were treated for 48 hours with 8 nmol/L GW1843 and live, blebbed, and dead cells purified on histopaque gradients (described in Table 1). Cells from each fraction were placed back into culture (0.5 × 106 cells/mL) in RPMI 1640 media with 10% fetal calf serum in the presence or absence of 20 μmol/L thymidine. An equal volume of media ± thymidine was added at 48 hours. Aliquots of the cells were counted at 48 and 96 hours to determine cell number. Results represent the average of three separate experiments and are presented as mean ± SEM.
Abbreviation: ND, not determined.
When examined visually 95.5 ± 1.2% of the cells were dead determined by trypan blue exclusion.
When examined visually 68.9 ± 6.7% of the cells were live determined by trypan blue exclusion.