Table 1. Oligonucleotides Used in RT or... | American Society of Hematology

Table 1.

Oligonucleotides Used in RT or PCR Experiments

Primer Sequence	Location
;I. GC (GAATTC) CTCTGAAGGTTCCAGAATCGATAG	S	Anchor^-150
;II. AGAAGTGATTTGTTCGAGGGTC	S	^-151
;III. GGACCCTCAGCCCTGGACC	AS	^‡
;IV. CCAGAACGCGGTCGGCCCG	S	61-79
;V. TTCTCTGAGTTCTTCCTGAGC	AS	680-660
;VI. AGCAGTATGAAAGTACCATCGG	S	1558-1579
;VII. GTTTACTTGCTGTACGCTCG	AS	1800-1781
;VIII. CTGTCGATTCGGCAGACCGA	S	1831-1850
;IX. TCTGTGCTTTAGGGACCACTG	AS	1953-1933
;X. GC (GAATTC) GATCTCCTCTTGACTCTTG	AS	2180-2162
;XI. GCTCACTGATGCTGGCATGATG	AS	2208-2187
;XII. GC (GAATTC) GGTGAGTGAGTGGATAAGCGT	AS	2284-2264
XIII. AGAGCCCACAGAAGCATGGA	S	2012-2031
;XIV. TCACTTTTCACAGCATTGGCATTATCT	AS	2392-2366
;XV. GACAAGAGTCAAGAGGAGATC	S	2160-2180
;XVI. AAGGGCAGAGTTGGGGTAGG	AS	2774-2755

Primer Sequence	Location
;I. GC (GAATTC) CTCTGAAGGTTCCAGAATCGATAG	S	Anchor^-150
;II. AGAAGTGATTTGTTCGAGGGTC	S	^-151
;III. GGACCCTCAGCCCTGGACC	AS	^‡
;IV. CCAGAACGCGGTCGGCCCG	S	61-79
;V. TTCTCTGAGTTCTTCCTGAGC	AS	680-660
;VI. AGCAGTATGAAAGTACCATCGG	S	1558-1579
;VII. GTTTACTTGCTGTACGCTCG	AS	1800-1781
;VIII. CTGTCGATTCGGCAGACCGA	S	1831-1850
;IX. TCTGTGCTTTAGGGACCACTG	AS	1953-1933
;X. GC (GAATTC) GATCTCCTCTTGACTCTTG	AS	2180-2162
;XI. GCTCACTGATGCTGGCATGATG	AS	2208-2187
;XII. GC (GAATTC) GGTGAGTGAGTGGATAAGCGT	AS	2284-2264
XIII. AGAGCCCACAGAAGCATGGA	S	2012-2031
;XIV. TCACTTTTCACAGCATTGGCATTATCT	AS	2392-2366
;XV. GACAAGAGTCAAGAGGAGATC	S	2160-2180
;XVI. AAGGGCAGAGTTGGGGTAGG	AS	2774-2755

The location of nucleotides is given within the sequence published by Conboy et al.15

Abbreviations: S, sense strand; AS, antisense strand.

Sequence complementary to the anchor used in the Amplifinder kit (Clontech).

‡Genomic sequences isolated from the W27 clone²³; they are located upstream and downstream from exon 1, respectively.23