Effects of TPO on the Growth of JCML GM Progenitors in Serum-Deprived Single-Cell Cultures
| Cases . | TPO . | None . | SCF . | GM-CSF . | SCF + GM-CSF . | GFs . |
|---|---|---|---|---|---|---|
| JCML | ||||||
| Case 1 | − | 0 | 0 | 0 | 11 ± 2 | 15 ± 2 |
| + | 0 | 1 ± 1 | 0 | 10 ± 1 | 14 ± 1 | |
| Case 2 | − | 0 | 0 | 0 | 12 ± 2 | 11 ± 1 |
| + | 0 | 0 | 0 | 10 ± 2 | 10 ± 1 | |
| Case 3 | − | 0 | 0 | 0 | 10 ± 1 | 10 ± 1 |
| + | 0 | 0 | 0 | 9 ± 1 | 9 ± 1 | |
| Case 4 | − | 0 | 0 | 0 | 11 ± 2 | 14 ± 1 |
| + | 0 | 0 | 0 | 12 ± 1 | 13 ± 1 | |
| Case 5 | − | 0 | 0 | 0 | 12 ± 1 | 11 ± 2 |
| + | 0 | 1 ± 1 | 0 | 10 ± 2 | 12 ± 4 | |
| Normal | ||||||
| Control 1 | − | 0 | 0 | 0 | 1 ± 1 | 11 ± 2 |
| + | 0 | 0 | 0 | 7 ± 2* | 10 ± 0 | |
| Control 2 | − | 0 | 0 | 0 | 2 ± 1 | 11 ± 2 |
| + | 0 | 0 | 0 | 7 ± 2† | 12 ± 2 | |
| Control 3 | − | 0 | 0 | 0 | 2 ± 1 | 13 ± 2 |
| + | 0 | 0 | 0 | 6 ± 1† | 11 ± 2 |
| Cases . | TPO . | None . | SCF . | GM-CSF . | SCF + GM-CSF . | GFs . |
|---|---|---|---|---|---|---|
| JCML | ||||||
| Case 1 | − | 0 | 0 | 0 | 11 ± 2 | 15 ± 2 |
| + | 0 | 1 ± 1 | 0 | 10 ± 1 | 14 ± 1 | |
| Case 2 | − | 0 | 0 | 0 | 12 ± 2 | 11 ± 1 |
| + | 0 | 0 | 0 | 10 ± 2 | 10 ± 1 | |
| Case 3 | − | 0 | 0 | 0 | 10 ± 1 | 10 ± 1 |
| + | 0 | 0 | 0 | 9 ± 1 | 9 ± 1 | |
| Case 4 | − | 0 | 0 | 0 | 11 ± 2 | 14 ± 1 |
| + | 0 | 0 | 0 | 12 ± 1 | 13 ± 1 | |
| Case 5 | − | 0 | 0 | 0 | 12 ± 1 | 11 ± 2 |
| + | 0 | 1 ± 1 | 0 | 10 ± 2 | 12 ± 4 | |
| Normal | ||||||
| Control 1 | − | 0 | 0 | 0 | 1 ± 1 | 11 ± 2 |
| + | 0 | 0 | 0 | 7 ± 2* | 10 ± 0 | |
| Control 2 | − | 0 | 0 | 0 | 2 ± 1 | 11 ± 2 |
| + | 0 | 0 | 0 | 7 ± 2† | 12 ± 2 | |
| Control 3 | − | 0 | 0 | 0 | 2 ± 1 | 13 ± 2 |
| + | 0 | 0 | 0 | 6 ± 1† | 11 ± 2 |
Data are the means ± SD of triplicate 96-well culture plates containing a single cell per well. TPO, 10 ng/mL; SCF, 10 ng/mL; GM-CSF, 10 ng/mL; GFs, combination of SCF (10 ng/mL), GM-CSF (10 ng/mL), IL-3 (100 U/mL), and G-CSF (10 ng/mL).
Significantly different from SCF + GM-CSF (*P < .01, †P < .05).