NO Produced by Activated BEC Induces Apoptosis in CD95-Sensitive Human Lymphoid Cell Lines
| Targets . | % Apoptosis . | |||||
|---|---|---|---|---|---|---|
| BEC . | BEC + TNF . | BEC + TNF + NMMA . | BEC + TNF + CHX . | BEC + TNF + zVAD . | BEC + TNF + F(ab′)2 Anti-CD95 . | |
| APO-S Jurkat | 3.6/1.7 | 28.7/30.8 | 1.5/1.4 | 14.5/16.9 | 4.4/1.4 | 16.6/16.2 |
| APO-R Jurkat | 1.7/1.7 | 10.2/10.2 | 1.2/3.3 | — | — | — |
| BJAB | 2.3/1.2 | 23.8/23.1 | 2.3/3.2 | 1.4/3.4 | 3.7/4.8 | 13.3/13.8 |
| BJAB FADD-DN | 1.5/1.1 | 6.5/4.1 | 1.7/2.7 | — | — | — |
| Targets . | % Apoptosis . | |||||
|---|---|---|---|---|---|---|
| BEC . | BEC + TNF . | BEC + TNF + NMMA . | BEC + TNF + CHX . | BEC + TNF + zVAD . | BEC + TNF + F(ab′)2 Anti-CD95 . | |
| APO-S Jurkat | 3.6/1.7 | 28.7/30.8 | 1.5/1.4 | 14.5/16.9 | 4.4/1.4 | 16.6/16.2 |
| APO-R Jurkat | 1.7/1.7 | 10.2/10.2 | 1.2/3.3 | — | — | — |
| BJAB | 2.3/1.2 | 23.8/23.1 | 2.3/3.2 | 1.4/3.4 | 3.7/4.8 | 13.3/13.8 |
| BJAB FADD-DN | 1.5/1.1 | 6.5/4.1 | 1.7/2.7 | — | — | — |
BEC were treated with human TNF-α (1 nmol/L final concentration) or TNF-α and NMMA (0.5 mmol/L final concentration), then washed twice with medium and cocultured for 8 hours with neoplastic lymphoid cells (effector/target ratio, 25:1). The latter cells were then collected and DNA release assay was performed. For inhibition experiments, lymphoid cells were treated for the whole period of coculture with 2 μg/mL CHX, or 20 μmol/L zVAD-fmk (zVAD) or 1 μg/mL F(ab′)2anti-CD95 blocking antibody. Percentage of apoptotic cells induced by these inhibitors alone was in all the cases less than 5%. Also, the percentage of nonspecific apoptosis in the control cells (not coculture with BEC) was in all the cases less than 7%. Data from two independent experiments are presented.