Table 1.

Sensitivity of Different Human Lymphoid Cell Lines Toward Apoptotic Cell Death Mediated by Anti-CD95 MoAb and NO

NO-Sensitive NO-Resistant 
CD95-Dependent CD95-Independent  
Cell Lines % Apoptosis  Cell Lines  % Apoptosis  Cell Lines  % Apoptosis 
+Anti-CD95  +GTN  +Anti-CD95  +GTN  +Anti-CD95  +GTN 
APO-S Jurkat  92.3  32.7  BL60   0.5  42.4 APO-R Jurkat   0.5  7.8  
CEM  57.9  29.0  K50 35.1  33.2  DN FADD BJAB  11.2  2.5  
T-ALL  40.4 26.5  
BJAB  83.1  29.2        
NO-Sensitive NO-Resistant 
CD95-Dependent CD95-Independent  
Cell Lines % Apoptosis  Cell Lines  % Apoptosis  Cell Lines  % Apoptosis 
+Anti-CD95  +GTN  +Anti-CD95  +GTN  +Anti-CD95  +GTN 
APO-S Jurkat  92.3  32.7  BL60   0.5  42.4 APO-R Jurkat   0.5  7.8  
CEM  57.9  29.0  K50 35.1  33.2  DN FADD BJAB  11.2  2.5  
T-ALL  40.4 26.5  
BJAB  83.1  29.2        

The different cell lines were plated at a density of 3 × 105 cells/mL and incubated for 16 hours in the presence or absence of 100 ng/mL agonistic anti-CD95 MoAb. Cells were incubated under the same conditions with 0.2 mmol/L GTN (APO-S, APO-R, and freshly isolated PBMC from a patient with T-ALL or 0.25 mmol/L GTN (CEM, BJAB, BJAB FADD-DN, BL60, and K50) for 24 hours. The percentage of apoptotic cells was determined by the DNA-release assay. Data shown are representative of three independent experiments (except freshly isolated leukemic lymphocytes). SD was less than 10%.

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