2° CFC and LTC-IC Frequencies Measured Before and After 5-FU Exposure
| Plating Density (103 per well) . | 2° CFC Frequency (per 105 cells) . | LTC-IC Frequency (per 105 cells) . | N . |
|---|---|---|---|
| Fresh BM | |||
| 20 | 22.3 | 14.7 | 10 |
| 10 | 24.1 | ||
| 5 | 30.5 | ||
| 2.5 | 50.1 | ||
| Post–5-FU Exposure | |||
| 20 | 18.5 | 7.2 | 10 |
| 10 | 19.8 | ||
| 5 | 15.8 | ||
| 2.5 | 18.3 |
| Plating Density (103 per well) . | 2° CFC Frequency (per 105 cells) . | LTC-IC Frequency (per 105 cells) . | N . |
|---|---|---|---|
| Fresh BM | |||
| 20 | 22.3 | 14.7 | 10 |
| 10 | 24.1 | ||
| 5 | 30.5 | ||
| 2.5 | 50.1 | ||
| Post–5-FU Exposure | |||
| 20 | 18.5 | 7.2 | 10 |
| 10 | 19.8 | ||
| 5 | 15.8 | ||
| 2.5 | 18.3 |
The frequency of 2° CFC (expressed as number per 105MNCs) was measured from 10 BM MNC samples using 20 replicate cultures at each of the shown plating densities in 96-well plates both before and after 1 to 2 hours of exposure to 250 μg/mL of 5-FU. In addition, LDA was used to determine the frequency of LTC-IC (expressed as number per 105 MNCs) for each group of densities that were performed in parallel.