2° CFC Frequencies Measured at Different Assay Plating Densities
Plating Density (103 per well)
.
2° CFC Frequency (per 105 cells)
.
N
.
500
4.2
4
250
11.1
4
250
10.7
7
100
23.3
7
30
50.2
7
100
34.3
26
50
44.2
26
20
63.8
26
Plating Density (103 per well)
.
2° CFC Frequency (per 105 cells)
.
N
.
500
4.2
4
250
11.1
4
250
10.7
7
100
23.3
7
30
50.2
7
100
34.3
26
50
44.2
26
20
63.8
26
The frequency of 2° CFC (expressed as number per 105MNCs) was measured from 37 BM MNC samples using three to six replicate cultures at each of the shown plating densities in 24-well plates.