Multilineage Colony Formation of Nonadherent Cells Produced by LTBMCs
Examined Time (wk) . | No. Colonies . | No. Erythroid Bursts . | ||
---|---|---|---|---|
Control . | TN-C DM . | Control . | TN-C DM . | |
7-wk-old mice | ||||
0 | 130 ± 6 | 85 ± 5-150 | 10 ± 2 | 7 ± 2-150 |
2 | 145 ± 5 | 69 ± 1-150 | 8 ± 1 | 6 ± 1† |
4 | 89 ± 1 | 30 ± 2-150 | 8 ± 1 | 3 ± 1† |
8 | 41 ± 2 | 21 ± 6-150 | 7 ± 1 | 4 ± 2† |
12 | 59 ± 4 | 44 ± 2-150 | 4 ± 0 | 3 ± 0 |
16 | 37 ± 1 | 20 ± 1-150 | 2 ± 0 | 1 ± 0 |
50-wk-old mice | ||||
0 | 61 ± 6 | 40 ± 3-150 | 4 ± 1 | 3 ± 1† |
8 | 40 ± 3 | 16 ± 1† | 3 ± 1 | 1 ± 0† |
Examined Time (wk) . | No. Colonies . | No. Erythroid Bursts . | ||
---|---|---|---|---|
Control . | TN-C DM . | Control . | TN-C DM . | |
7-wk-old mice | ||||
0 | 130 ± 6 | 85 ± 5-150 | 10 ± 2 | 7 ± 2-150 |
2 | 145 ± 5 | 69 ± 1-150 | 8 ± 1 | 6 ± 1† |
4 | 89 ± 1 | 30 ± 2-150 | 8 ± 1 | 3 ± 1† |
8 | 41 ± 2 | 21 ± 6-150 | 7 ± 1 | 4 ± 2† |
12 | 59 ± 4 | 44 ± 2-150 | 4 ± 0 | 3 ± 0 |
16 | 37 ± 1 | 20 ± 1-150 | 2 ± 0 | 1 ± 0 |
50-wk-old mice | ||||
0 | 61 ± 6 | 40 ± 3-150 | 4 ± 1 | 3 ± 1† |
8 | 40 ± 3 | 16 ± 1† | 3 ± 1 | 1 ± 0† |
Colonies (>50 cells) were counted at day 14. Visualized hemoglobinized colonies were counted as erythroid bursts (BFU-E). Data represented as mean colony number ± SD per 5 × 104nonadherent cells from triplicate cultures. Progenitor cell assays were performed using BM cells before introduction of LTBMCs (week 0) and nonadherent cells from murine LTBMCs at appropriate intervals of cultures (week 2-16).
Abbreviation: TN-C DM, tenascin-C–deficient mutant mice.
P < .01, †P < .05, according to Student'st-test. Two additional experiments gave comparable results.