Effect of Antioxidants on Membrane TNFα and TNF-RII Expression on Stimulated T Cells
| . | . | Compounds . | Range of Concentrations . | IC50 . |
|---|---|---|---|---|
| With SH | NAC | .1-20 mmol/L | 12 mmol/L | |
| NAC + 100 μmol/L BSO | .1-20 mmol/L | 12 mmol/L | ||
| GSH | .1-20 mmol/L | 12 mmol/L | ||
| Cysteine | .1-20 mmol/L | 8 mmol/L | ||
| Captopril | 13 mmol/L | |||
| DTT | .1-1 mmol/L | 1 μmol/L | ||
| MPA | .5-20 mmol/L | 5 μmol/L | ||
| Antioxidant | Without SH | SOD | 10-400 U/mL | — |
| Catalase | 10-400 U/mL | — | ||
| Desferrioxamine | 1-100 mmol/L | — | ||
| Vitamin C | .01-10 mmol/L | — | ||
| L-carnithine | 1-25 mmol/L | — | ||
| Nonfree thiol residue | GS-SG | .5-20 mmol/L | — | |
| S-methyl cysteine | .5-20 mmol/L | — | ||
| Methionine | .5-20 mmol/L | — |
| . | . | Compounds . | Range of Concentrations . | IC50 . |
|---|---|---|---|---|
| With SH | NAC | .1-20 mmol/L | 12 mmol/L | |
| NAC + 100 μmol/L BSO | .1-20 mmol/L | 12 mmol/L | ||
| GSH | .1-20 mmol/L | 12 mmol/L | ||
| Cysteine | .1-20 mmol/L | 8 mmol/L | ||
| Captopril | 13 mmol/L | |||
| DTT | .1-1 mmol/L | 1 μmol/L | ||
| MPA | .5-20 mmol/L | 5 μmol/L | ||
| Antioxidant | Without SH | SOD | 10-400 U/mL | — |
| Catalase | 10-400 U/mL | — | ||
| Desferrioxamine | 1-100 mmol/L | — | ||
| Vitamin C | .01-10 mmol/L | — | ||
| L-carnithine | 1-25 mmol/L | — | ||
| Nonfree thiol residue | GS-SG | .5-20 mmol/L | — | |
| S-methyl cysteine | .5-20 mmol/L | — | ||
| Methionine | .5-20 mmol/L | — |
T cells were stimulated for 16 hours with PMA plus ionomycin in the presence of different concentrations of the compounds. Membrane TNFα and TNF-RII expression was evaluated by FACS analysis. Results are expressed as an IC50 value (concentration inducing 50% increase of expression); means uneffective.