Effect of Inhibitors of Cell Activation and/or Cell Proliferation on the In Vitro Deletion of TCR Vβ8.1 TG DP Thymocytes
| . | Thymocytes . | +BALB.D2 B Cells . | % Deletion . | n . |
|---|---|---|---|---|
| Untreated culture | 31.300 ± 4.700 | 7.700 ± 2.500 | 74 ± 9 | 5 |
| + PKC inhibitor | 38.600 ± 6.200 | 33.900 ± 7.500 | 14 ± 10 | 4 |
| + Calcineurin inhibitor | 24.100 ± 3.000 | 14.600 ± 3.200 | 40 ± 8 | 4 |
| + Cell-cycle inhibitor | 34.300 ± 3.800 | 8.900 ± 3.000 | 73 ± 10 | 4 |
| . | Thymocytes . | +BALB.D2 B Cells . | % Deletion . | n . |
|---|---|---|---|---|
| Untreated culture | 31.300 ± 4.700 | 7.700 ± 2.500 | 74 ± 9 | 5 |
| + PKC inhibitor | 38.600 ± 6.200 | 33.900 ± 7.500 | 14 ± 10 | 4 |
| + Calcineurin inhibitor | 24.100 ± 3.000 | 14.600 ± 3.200 | 40 ± 8 | 4 |
| + Cell-cycle inhibitor | 34.300 ± 3.800 | 8.900 ± 3.000 | 73 ± 10 | 4 |
Data are indicated as the mean ± SD of the absolute number of DP thymocytes per well obtained after culture of TCR Vβ8.1 TG thymocytes alone or with BALB.D2 B cells for 60 hours, in the absence or presence of the indicated inhibitors. A representative experiment is illustrated in Fig 4.