Stimulatory Effect of Linolenic Acid on Proliferation of P-HSCs in Long-Term Culture
| . | Per Flask . | |
|---|---|---|
| . | No. of Cobblestone Colonies . | No. of Non-Ad. Cells (×104) . |
| Control | 4.3 ± 0.9 | 1.7 ± 0.2 |
| Pre-incubation | ||
| 1 μg/mL | 33.7 ± 5.26-150 | 9.4 ± 1.46-150 |
| 5 μg/mL | 49.3 ± 9.36-150 | 12.2 ± 2.16-150 |
| Co-culture | ||
| 1 μg/mL | 43.7 ± 5.86-150 | 12.1 ± 1.56-150 |
| 5 μg/mL | 6.3 ± 2.86-151 | 1.7 ± 0.26-151 |
| . | Per Flask . | |
|---|---|---|
| . | No. of Cobblestone Colonies . | No. of Non-Ad. Cells (×104) . |
| Control | 4.3 ± 0.9 | 1.7 ± 0.2 |
| Pre-incubation | ||
| 1 μg/mL | 33.7 ± 5.26-150 | 9.4 ± 1.46-150 |
| 5 μg/mL | 49.3 ± 9.36-150 | 12.2 ± 2.16-150 |
| Co-culture | ||
| 1 μg/mL | 43.7 ± 5.86-150 | 12.1 ± 1.56-150 |
| 5 μg/mL | 6.3 ± 2.86-151 | 1.7 ± 0.26-151 |
MS-5 were pre-incubated with linolenic acid (1 or 5 μg/mL) for 1 day and washed two times. P-HSC were cultured on the MS-5 and the culture medium was replaced weekly with fresh medium. Six weeks later, the numbers of cobblestone colonies and nonadherent cells per flask were counted. Mean ± SE.
Different from control (P < .05).
Not significant.