BCL2 Gene Amplification, Densitometric Values of all the Cases (t1/t2), t(14; 18), BCL2 Gene Rearrangement, DNA Copy Number Changes at 18q, and BCL2 Protein Expression in 26 Diffuse Large B-Cell Lymphomas
| Case No. . | t(14; 18) Detected by Karyotype Analysis . | BCL2 Rearrangement by PCR . | BCL2 Amplification by Southern Blot Hybridization . | BLC2/p105-153A (t1/t2) . | DNA Copy Number Changes at 18q by CGH . | BCL2 Protein Expression WB/IH . |
|---|---|---|---|---|---|---|
| 1 | − | − | + | 1.6 | ++ | +/++ |
| 2 | − | − | + | 1.4 | + | +/++ |
| 3 | − | − | + | 1.4 | + | +/++ |
| 4 | − | − | + | 1.9 | ++ | +/++ |
| 5 | − | − | + | 1.6 | + | +/++ |
| 6 | − | − | + | 1.8 | ++ | +/++ |
| 7 | − | ns | + | 1.7 | ++ | +/++ |
| 8 | − | ns | + | 2.1 | ++ | +/++ |
| 9 | + | + | − | 1.1 | n | +/+ |
| 10 | + | − | − | 0.7 | n | +/++ |
| 11 | ns | + | − | 0.9 | n | +/++ |
| 12 | − | − | − | 1.0 | n | −/− |
| 13 | ns | − | − | 1.1 | n | +/++ |
| 14 | − | − | − | 1.0 | n | −/+ |
| 15 | − | − | − | 0.9 | n | −/+ |
| 16 | − | − | − | 1.3 | n | ns/++ |
| 17 | − | − | − | 1.1 | n | −/+ |
| 18 | ns | − | − | 1.2 | n | ns/++ |
| 19 | ns | − | − | 1.1 | n | −/+ |
| 20 | − | − | − | 1.0 | n | −/+ |
| 21 | ns | − | − | 1.3 | n | ns/− |
| 22 | − | − | − | 1.3 | n | −/− |
| 23 | − | − | − | 0.9 | n | −/− |
| 24 | − | − | − | 0.6 | − | −/− |
| 25 | ns | − | − | 0.8 | n | −/− |
| 26 | ns | − | − | 0.8 | n | −/− |
| Case No. . | t(14; 18) Detected by Karyotype Analysis . | BCL2 Rearrangement by PCR . | BCL2 Amplification by Southern Blot Hybridization . | BLC2/p105-153A (t1/t2) . | DNA Copy Number Changes at 18q by CGH . | BCL2 Protein Expression WB/IH . |
|---|---|---|---|---|---|---|
| 1 | − | − | + | 1.6 | ++ | +/++ |
| 2 | − | − | + | 1.4 | + | +/++ |
| 3 | − | − | + | 1.4 | + | +/++ |
| 4 | − | − | + | 1.9 | ++ | +/++ |
| 5 | − | − | + | 1.6 | + | +/++ |
| 6 | − | − | + | 1.8 | ++ | +/++ |
| 7 | − | ns | + | 1.7 | ++ | +/++ |
| 8 | − | ns | + | 2.1 | ++ | +/++ |
| 9 | + | + | − | 1.1 | n | +/+ |
| 10 | + | − | − | 0.7 | n | +/++ |
| 11 | ns | + | − | 0.9 | n | +/++ |
| 12 | − | − | − | 1.0 | n | −/− |
| 13 | ns | − | − | 1.1 | n | +/++ |
| 14 | − | − | − | 1.0 | n | −/+ |
| 15 | − | − | − | 0.9 | n | −/+ |
| 16 | − | − | − | 1.3 | n | ns/++ |
| 17 | − | − | − | 1.1 | n | −/+ |
| 18 | ns | − | − | 1.2 | n | ns/++ |
| 19 | ns | − | − | 1.1 | n | −/+ |
| 20 | − | − | − | 1.0 | n | −/+ |
| 21 | ns | − | − | 1.3 | n | ns/− |
| 22 | − | − | − | 1.3 | n | −/− |
| 23 | − | − | − | 0.9 | n | −/− |
| 24 | − | − | − | 0.6 | − | −/− |
| 25 | ns | − | − | 0.8 | n | −/− |
| 26 | ns | − | − | 0.8 | n | −/− |
Abbreviations: − denotes negative and + positive result for t(14;18), BCL2 rearrangement, amplification, or protein expression. In immunohistochemistry ++ denotes staining in 80-100% of the cells, + in 50% to 80% of the cells, and − denotes BCL2 expression in less than 10% of the cells. Cases not studied have been marked with ns. The cases with a gain or a high-level amplification of chromosome 18q in CGH analysis have been marked with + and ++, respectively. Normal chromosome 18 in a copy number karyotype assessed by CGH has been marked with n, and the loss of the chromosome with −. WB, Western Blot; IH, immunohistochemistry.