In Vitro Differentiation of Leukemia Cells From Patients by Am80 and Their Clinical Response to Am80 Therapy
| Patient . | Month . | Blast % . | In Vitro Response (%)3-150 . | |||||
|---|---|---|---|---|---|---|---|---|
| . | From Last . | in . | Am80 . | ATRA . | Clinical . | Day to . | ||
| . | ATRA . | Marrow . | 10−8 mol/L . | 10−6 mol/L . | 10−8 mol/L . | 10−6 mol/L . | Response . | CR . |
| 1 | 30 | 23 | 83 | 92.5 | 77.5 | 94.5 | CR | 20 |
| 2 | 18 | 97 | 70.5 | NT | 74.5 | NT | CR | 46 |
| 3 | 25 | 14 | 72 | 88 | 75.5 | 92.5 | CR | 37 |
| 4 | 22 | 26 | 47.5 | NT | 58 | NT | CR | 41 |
| 5 | 24 | 92 | 75 | 84.5 | 16 | 24.5 | CR | 43 |
| 6 | 18 | 88 | 56.5 | 79.5 | 20.5 | 40.5 | CR | 46 |
| 7 | 22 | 84 | 53.5 | 83 | 20.5 | 39 | CR | 28 |
| 8 | 25 | 14 | 40.5 | 66 | 35 | 60.5 | CR | 40 |
| 9 | 45 | 89 | 34 | 66.5 | 28.5 | 60.5 | CR | 58 |
| 10 | 3 | 67 | 30 | 59.5 | 27.5 | 63 | F | |
| 11 | 14 | 62 | 14 | 63.5 | 15 | 67 | F | |
| 12 | 14 | 96 | 11.5 | 29 | 12 | 32.5 | F | |
| 13 | 20 | 88 | 11 | 30.5 | 8 | 13.5 | F | |
| 14 | 25 | 44 | 9 | 13.5 | 10.5 | 12 | F | |
| 15 | 27 | 82 | 4.5 | 12 | 6.5 | 10.5 | F | |
| 16 | 2 | 25 | 3 | NT | 4.5 | NT | F | |
| 17 | 30 | 96 | 2.5 | 5 | 2 | 2 | F | |
| Patient . | Month . | Blast % . | In Vitro Response (%)3-150 . | |||||
|---|---|---|---|---|---|---|---|---|
| . | From Last . | in . | Am80 . | ATRA . | Clinical . | Day to . | ||
| . | ATRA . | Marrow . | 10−8 mol/L . | 10−6 mol/L . | 10−8 mol/L . | 10−6 mol/L . | Response . | CR . |
| 1 | 30 | 23 | 83 | 92.5 | 77.5 | 94.5 | CR | 20 |
| 2 | 18 | 97 | 70.5 | NT | 74.5 | NT | CR | 46 |
| 3 | 25 | 14 | 72 | 88 | 75.5 | 92.5 | CR | 37 |
| 4 | 22 | 26 | 47.5 | NT | 58 | NT | CR | 41 |
| 5 | 24 | 92 | 75 | 84.5 | 16 | 24.5 | CR | 43 |
| 6 | 18 | 88 | 56.5 | 79.5 | 20.5 | 40.5 | CR | 46 |
| 7 | 22 | 84 | 53.5 | 83 | 20.5 | 39 | CR | 28 |
| 8 | 25 | 14 | 40.5 | 66 | 35 | 60.5 | CR | 40 |
| 9 | 45 | 89 | 34 | 66.5 | 28.5 | 60.5 | CR | 58 |
| 10 | 3 | 67 | 30 | 59.5 | 27.5 | 63 | F | |
| 11 | 14 | 62 | 14 | 63.5 | 15 | 67 | F | |
| 12 | 14 | 96 | 11.5 | 29 | 12 | 32.5 | F | |
| 13 | 20 | 88 | 11 | 30.5 | 8 | 13.5 | F | |
| 14 | 25 | 44 | 9 | 13.5 | 10.5 | 12 | F | |
| 15 | 27 | 82 | 4.5 | 12 | 6.5 | 10.5 | F | |
| 16 | 2 | 25 | 3 | NT | 4.5 | NT | F | |
| 17 | 30 | 96 | 2.5 | 5 | 2 | 2 | F | |
Abbreviations: NT, not tested; F, failure.
The in vitro differentiation of leukemia cells was predicted by the restoration of PML and PML-RAR-α subcellular localization from diffuse fine-granular patterns to normal speckled patterns using an immunostaining method. We examined 100 cells and determined the percentage of cells with the speckled staining. When an APL cell showed a full restoration to a normal speckled pattern, we counted it as 1. When it showed incomplete speckled staining with fine granules and/or cytoplasmic staining, we counted it as 0.5.