Percent Change in Cell Number in the Absence and Presence of PMA
.
iNOS
.
Empty Vector
.
Without PMA
132 ± 35
123 ± 26
PMA (100 nmol/L)
−25 ± 16
−22 ± 14
.
iNOS
.
Empty Vector
.
Without PMA
132 ± 35
123 ± 26
PMA (100 nmol/L)
−25 ± 16
−22 ± 14
Cells were incubated for 2 days in 50 mL of AMEM containing 10% FCS in 180 cm2 flasks. Cells were then suspended with 1 mmol/L EDTA in PBS for 30 minutes before counting (mean percent change ± SE, n = 4).