Table 4.

Summary of the Sequence Analysis and the Assessment of Intraclonal Heterogeneity of the Three Cases

Patient Biopsy VH Family Germ-line (GL) Donor D-Segment Gene JH-Gene TumorDerived/ Total B-Cell Sequences Intraclonal Heterogeneity No. of Different but Clonally Related Sequences % Homology of Dominant Sequence to GLRange of % Homology to GL
BA  1F VH1  VI-4.1.b  D5  JH4b  23/25 Yes  16  93.1  92.4-93.8  
 2P  VH1 VI-4.1.b  D5  JH4b  8/24  Yes  3 93.4  91.6-93.4  
EJ  1F  VH3  V3-7 D21-9 JH3a 21/28 Yes 4 93.7 93.7-94.4 
 2F VH3 V3-7 D21-9 JH3a 16/19 Yes 11 94.8 94.3-94.8 
 3F VH3 V3-7 D21-9 JH3a 23/25 Yes 9 94.2 92.4-95.5 
BR 1P VH3 V3-30.5 DXP5a JH4b 9/10 Yes 4 84.6 83.6-84.6 
 2F VH3 V3-30.5 DXP5a JH4b 9/12 No 1 86.7* Homogeneous 
Patient Biopsy VH Family Germ-line (GL) Donor D-Segment Gene JH-Gene TumorDerived/ Total B-Cell Sequences Intraclonal Heterogeneity No. of Different but Clonally Related Sequences % Homology of Dominant Sequence to GLRange of % Homology to GL
BA  1F VH1  VI-4.1.b  D5  JH4b  23/25 Yes  16  93.1  92.4-93.8  
 2P  VH1 VI-4.1.b  D5  JH4b  8/24  Yes  3 93.4  91.6-93.4  
EJ  1F  VH3  V3-7 D21-9 JH3a 21/28 Yes 4 93.7 93.7-94.4 
 2F VH3 V3-7 D21-9 JH3a 16/19 Yes 11 94.8 94.3-94.8 
 3F VH3 V3-7 D21-9 JH3a 23/25 Yes 9 94.2 92.4-95.5 
BR 1P VH3 V3-30.5 DXP5a JH4b 9/10 Yes 4 84.6 83.6-84.6 
 2F VH3 V3-30.5 DXP5a JH4b 9/12 No 1 86.7* Homogeneous 

*The frozen biopsy specimen BR-2F yielded longer sequence than the earlier biopsy 1P, with comparatively fewer mutations in FWR1. This leads to an apparent increase of homology to the germline sequence in the later biopsy.

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