Serine-Threonine and Tyrosine Kinase Inhibitors Block CD44DL
| Inhibitor . | Concentration . | % Inhibition of Lysis . | ||
|---|---|---|---|---|
| . | . | CD44DL . | NK . | ADCC . |
| Experiment no. 1 | ||||
| Staurosporine | 25 nmol/L | 100 | 74 | 100 |
| Staurosporine | 2.5 nmol/L | 39 | 23 | 75 |
| Experiment no. 2 | ||||
| Herbimycin A | 5.2 μmol/L | 99 | 90 | 98 |
| Herbimycin A | 0.52 μmol/L | 64 | 18 | 62 |
| Inhibitor . | Concentration . | % Inhibition of Lysis . | ||
|---|---|---|---|---|
| . | . | CD44DL . | NK . | ADCC . |
| Experiment no. 1 | ||||
| Staurosporine | 25 nmol/L | 100 | 74 | 100 |
| Staurosporine | 2.5 nmol/L | 39 | 23 | 75 |
| Experiment no. 2 | ||||
| Herbimycin A | 5.2 μmol/L | 99 | 90 | 98 |
| Herbimycin A | 0.52 μmol/L | 64 | 18 | 62 |
Activated LD-PBL were tested for the ability to mediate the indicated type of lysis at an E:T of 24:1, except for NK lysis (natural cytotoxicity) in experiment no. 1, which was measured at 5:1. The percentages of lysis in the absence of inhibitors for CD44DL, NK, and ADCC were 52%, 46%, and 30% for experiment no. 1, and 33%, 91%, and 32% for experiment no. 2. Similar percentages of inhibition were obtained at other E:T ratios used in these experiments. Both inhibitors were used in at least four separate experiments with similar results.