Table 1. Inhibition of Tie Release by... | American Society of Hematology

Table 1.

Inhibition of Tie Release by Protease Inhibitors and Chelation of Divalent Cations

Inhibitor	Concentration	Soluble Tie Release (% control)^*
Leupeptin	1 μg/mL	100
Benzamidine	1 mmol/L	92
TLCK	40 μg/mL	96
PMSF	500 μmol/L	107
Aprotinin	1 μg/mL	119
Pepstatin	1 μg/mL	87
NCO-700	100 μmol/L	66
Phosphoramidon	100 μg/mL	125
o-Phenanthroline	20 μmol/L	92
EDTA	100 μmol/L	103
EGTA	100 μmol/L	4
EGTA + Ca²⁺	100 μmol/L + 100 μmol/L	88
EGTA + Ca²⁺	100 μmol/L + 500 μmol/L	100

Inhibitor	Concentration	Soluble Tie Release (% control)^*
Leupeptin	1 μg/mL	100
Benzamidine	1 mmol/L	92
TLCK	40 μg/mL	96
PMSF	500 μmol/L	107
Aprotinin	1 μg/mL	119
Pepstatin	1 μg/mL	87
NCO-700	100 μmol/L	66
Phosphoramidon	100 μg/mL	125
o-Phenanthroline	20 μmol/L	92
EDTA	100 μmol/L	103
EGTA	100 μmol/L	4
EGTA + Ca²⁺	100 μmol/L + 100 μmol/L	88
EGTA + Ca²⁺	100 μmol/L + 500 μmol/L	100

HUVECs were pretreated with protease inhibitors, at the indicated concentrations, for 15 minutes before incubation with 10 ng/mL PMA for 1 hour. Supernatants were collected and assayed for soluble tie release by ELISA as described in Materials and Methods. Experiments with EDTA/EGTA were performed in PBS (−Ca²⁺/Mg²⁺) + 1% FBS instead of basal media. Results are expressed as % control, where 100% control represents soluble tie released in response to 10 ng/mL PMA in the absence of protease inhibitors or divalent cation chelators.

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