CD45 Isoform Subsets in Thymus-Bearing Hosts
| Thymus-Bearing Host . | Time After BMT . | CD4+ . | CD8+ . | ||
|---|---|---|---|---|---|
| . | . | CD45RA+ . | CD45RO+ . | CD45RA+ . | CD45RO+ . |
| (6) | 12 mo | 65 | 28 | 83 | 7 |
| (7) | 9 mo | 66 | 31 | 84 | 9 |
| (8) | 24 mo | 28 | 61 | 72 | 12 |
| (9) | 12 mo | 28 | 65 | 78 | 13 |
| Thymus-Bearing Host . | Time After BMT . | CD4+ . | CD8+ . | ||
|---|---|---|---|---|---|
| . | . | CD45RA+ . | CD45RO+ . | CD45RA+ . | CD45RO+ . |
| (6) | 12 mo | 65 | 28 | 83 | 7 |
| (7) | 9 mo | 66 | 31 | 84 | 9 |
| (8) | 24 mo | 28 | 61 | 72 | 12 |
| (9) | 12 mo | 28 | 65 | 78 | 13 |
T-cell subsets were quantified by FACS. To determine CD4+ and CD8+ cells, CD3+ cells were first identified by PerCP (FL3), gated, and then examined for expression of the CD4 and CD8 antigen by quadrant graphs of FL1 (anti-CD4, FITC) and FL2 (anti-CD8, PE). To determine CD45 isotype expression, CD4+ or CD8+ cells were first identified by PerCP (FL3), gated, and then examined by quadrant graphs for expression of FL1 (anti-CD45RA, FITC) and FL2 (anti-CD45RO, PE); numbers of CD45RA+ and CD45RO+ represent percentages of CD4+ and CD8+ cells. Absolute counts of CD4+ cells (expressed as cells per microliter) were 858/μL in host (6), 693/μL in host (7), 746/μL in host (8), and 123/μL in host (9). Absolute counts of CD8+ cells (expressed as cells per microliter) were 396/μL in host (6), 110/μL in host (7), 395/μL in host (8), and 32/μL in host (9).