Table 1. Primer Pairs and PCR Conditions... | American Society of Hematology

Table 1.

Primer Pairs and PCR Conditions for Screening of Mutation in H and Se Genes

	Name	Primers (5′-3′)	Inactivated Allele Detected	Annealing Temperature	Fragment Sizes	Enzyme

H gene	mk1	CTCGAATTCCTCAGCCTCAGAGCATTTG EcoRI	(h4)	60°C	1,170 bp	— (Eam1105 I)
	mk2	CTCAAGCTTCTACTTCAGAAAGTCTCCCTG HindIII
	mk3	CTATCTGCAGGTTATGCCTCAGCTCT	h1	62°C	78 bp	Xba I
	mk4	AACCAGTCCATGGCCTGCCGGAGG
	mk5	GCAACCACACCATTATGACCATTG	h2	62°C	116 bp	Xho I
	mk6	GATCTTCAGGAACTCAGAGTCTCG
	mk7	AAGGGTGTGGTGGGCGACAGGGCC	h3	62°C	101 bp	Apa I
	mk8	TCCATGCCGTTGCTGGTGACCACGA
	mk9	TTCACGACTGGATGCGGAGGTG	h5	62°C	126 bp	ApaLI
	mk10	GCAGGGTGAACTCTCTGCGGATC
	mk11	AACTTCACCCTGCCAGACTCTGAG	h5	62°C	125 bp	BstXI
	mk12	GGCTCTCAAGGCTTAGCCAATGTC
Se gene	tk1	CTCGAATTCGGGCCTCCATCTCCCAGCTAAC EcoRI		65°C	1,170 bp
	tk2	CTCAAGCTTGCTTCTCATGCCCGGGCACTC HindIII
	tk5	CAGGATCCCCTGGCAGAACTACCACATTAA	sej	65°C	98 bp	Ase I
	tk6	AGCAGGGGTAGCCGGTGAAGCGGACGTACT
	tk7	AACGACTGGATGGAGGAGGAATACCGCAGC	Se2, sej	65°C	80 bp	Alu I
	tk8	AAGGTCCAGGAGCAGGGGTAGCCGGTGAAG
Le gene	sn1	CTCGAATTCTAAGCAGGAGATTGTCATCACTGACC EcoRI		60°C	1,615 bp
	sn2	CTCAAGCTTCGTGCCGTGATGATCTCTCTGCAC HindIII
	sn3	CCATGGCGCCGCTGTCTGGCCGCCC	le1, le2	62°C	93 bp	Msp I
	sn4	AGTGGCATCGTCTCGGGACACACG
	sn6	CGCTCCTTCAGCTGGGCACTGGA	le2	60°C	109 bp	HindIII
	sn7	CGGCCTCTCAGGTGAACCAAGAAGCT
	sn8	ACTTGGAGCCACCCCCTAACTGCCA	le1	70°C	206 bp	Pvu II
	sn9	TGAGTCCGGCTTCCAGTTGGACACC

	Name	Primers (5′-3′)	Inactivated Allele Detected	Annealing Temperature	Fragment Sizes	Enzyme

H gene	mk1	CTCGAATTCCTCAGCCTCAGAGCATTTG EcoRI	(h4)	60°C	1,170 bp	— (Eam1105 I)
	mk2	CTCAAGCTTCTACTTCAGAAAGTCTCCCTG HindIII
	mk3	CTATCTGCAGGTTATGCCTCAGCTCT	h1	62°C	78 bp	Xba I
	mk4	AACCAGTCCATGGCCTGCCGGAGG
	mk5	GCAACCACACCATTATGACCATTG	h2	62°C	116 bp	Xho I
	mk6	GATCTTCAGGAACTCAGAGTCTCG
	mk7	AAGGGTGTGGTGGGCGACAGGGCC	h3	62°C	101 bp	Apa I
	mk8	TCCATGCCGTTGCTGGTGACCACGA
	mk9	TTCACGACTGGATGCGGAGGTG	h5	62°C	126 bp	ApaLI
	mk10	GCAGGGTGAACTCTCTGCGGATC
	mk11	AACTTCACCCTGCCAGACTCTGAG	h5	62°C	125 bp	BstXI
	mk12	GGCTCTCAAGGCTTAGCCAATGTC
Se gene	tk1	CTCGAATTCGGGCCTCCATCTCCCAGCTAAC EcoRI		65°C	1,170 bp
	tk2	CTCAAGCTTGCTTCTCATGCCCGGGCACTC HindIII
	tk5	CAGGATCCCCTGGCAGAACTACCACATTAA	sej	65°C	98 bp	Ase I
	tk6	AGCAGGGGTAGCCGGTGAAGCGGACGTACT
	tk7	AACGACTGGATGGAGGAGGAATACCGCAGC	Se2, sej	65°C	80 bp	Alu I
	tk8	AAGGTCCAGGAGCAGGGGTAGCCGGTGAAG
Le gene	sn1	CTCGAATTCTAAGCAGGAGATTGTCATCACTGACC EcoRI		60°C	1,615 bp
	sn2	CTCAAGCTTCGTGCCGTGATGATCTCTCTGCAC HindIII
	sn3	CCATGGCGCCGCTGTCTGGCCGCCC	le1, le2	62°C	93 bp	Msp I
	sn4	AGTGGCATCGTCTCGGGACACACG
	sn6	CGCTCCTTCAGCTGGGCACTGGA	le2	60°C	109 bp	HindIII
	sn7	CGGCCTCTCAGGTGAACCAAGAAGCT
	sn8	ACTTGGAGCCACCCCCTAACTGCCA	le1	70°C	206 bp	Pvu II
	sn9	TGAGTCCGGCTTCCAGTTGGACACC