Relative Repopulating Abilities of Late Fetal and Newborn Blood and Liver Cells
| Time (d) . | Amount Donor . | Late Fetal: . | Newborn . | Control BM . | |||
|---|---|---|---|---|---|---|---|
| . | . | Blood . | Liver . | Blood . | Liver (1) . | Liver (2) . | . |
| 62 | PE | 59 ± 1 | 56 ± 5 | 46 ± 8 | 65 ± 4 | 30 ± 10 | 53 ± 5 |
| RUE* | 1.4 | 38 | 0.6 | 56 | 34 | 11 | |
| PL | 60 ± 3 | 49 ± 6 | 47 ± 10 | 60 ± 6 | 24 ± 7 | 51 ± 5 | |
| RUL* | 1.5 | 29 | 0.6 | 45 | 25 | 10 | |
| 189 | PE | 76 ± 8 | 62 ± 2 | 70 ± 1 | 72 ± 7 | 35 ± 13 | 44 ± 5 |
| RUE* | 3.2 | 49 | 1.7 | 77 | 43 | 7.9 | |
| PL | 77 ± 5 | 56 ± 3 | 68 ± 4 | 68 ± 6 | 29 ± 9 | 38 ± 6 | |
| RUL* | 3.3 | 38 | 1.5 | 64 | 33 | 6.1 | |
| 306 | PE | 74 ± 6 | 65 ± 3 | 75 ± 7 | 74 ± 8 | 36 ± 12 | 34 ± 1 |
| RUE* | 2.8 | 56 | 2.2 | 85 | 45 | 5.2 | |
| PL | 80 ± 7 | 57 ± 4 | 69 ± 5 | 66 ± 4 | 33 ± 11 | 32 ± 10 | |
| RUL* | 4.0 | 40 | 1.6 | 58 | 39 | 4.7 | |
| Time (d) . | Amount Donor . | Late Fetal: . | Newborn . | Control BM . | |||
|---|---|---|---|---|---|---|---|
| . | . | Blood . | Liver . | Blood . | Liver (1) . | Liver (2) . | . |
| 62 | PE | 59 ± 1 | 56 ± 5 | 46 ± 8 | 65 ± 4 | 30 ± 10 | 53 ± 5 |
| RUE* | 1.4 | 38 | 0.6 | 56 | 34 | 11 | |
| PL | 60 ± 3 | 49 ± 6 | 47 ± 10 | 60 ± 6 | 24 ± 7 | 51 ± 5 | |
| RUL* | 1.5 | 29 | 0.6 | 45 | 25 | 10 | |
| 189 | PE | 76 ± 8 | 62 ± 2 | 70 ± 1 | 72 ± 7 | 35 ± 13 | 44 ± 5 |
| RUE* | 3.2 | 49 | 1.7 | 77 | 43 | 7.9 | |
| PL | 77 ± 5 | 56 ± 3 | 68 ± 4 | 68 ± 6 | 29 ± 9 | 38 ± 6 | |
| RUL* | 3.3 | 38 | 1.5 | 64 | 33 | 6.1 | |
| 306 | PE | 74 ± 6 | 65 ± 3 | 75 ± 7 | 74 ± 8 | 36 ± 12 | 34 ± 1 |
| RUE* | 2.8 | 56 | 2.2 | 85 | 45 | 5.2 | |
| PL | 80 ± 7 | 57 ± 4 | 69 ± 5 | 66 ± 4 | 33 ± 11 | 32 ± 10 | |
| RUL* | 4.0 | 40 | 1.6 | 58 | 39 | 4.7 | |
In experiment 1, percentages of erythrocytes (PE) of donor type or lymphocytes of donor type (PL) are given as mean ± SD; n = 3 to 4 in each group, except n = 14 to 16 for Liver (2). Recipients were given the following doses: Blood, 2 × 105 B6 standard BM cells, mixed with 2.0 (19 day fetal) or 2.75 (newborn) × 106 B6-HbbdGpi 1a donor nucleated blood cells; Liver, 3 × 106 B6 standard BM cells, mixed with 1 × 106 cells from B6-HbbdGpi 1a 19 day Fetal Liver or Newborn Liver (1), except recipients marked Newborn Liver (2) were given 8 × 105 B6 standard BM cells, mixed with 1 × 105 B6-HbbdGpi 1a newborn liver cells; Controls, 2 × 106 donor and standard adult BM cells, because doses of 1 to 4 million cells per recipient are standard when measuring repopulating abilities of fresh BM.23 Controls for recipient repopulation received 2 × 105 B6-HbbdGpi 1a adult BM cells only (values for PE, PL were 98 ± 4, 100 after 62 days, and 100, 100 at the two later time points, with n = 4.
RU per 106 donor cells injected, where 1.0 RU = repopulating ability of 1.0 × 105 standard cells.