Densitometric Measurement of Band 3, H2DIDS Binding Sites, and Sulfate Flux in Family MG
| Subject No. . | Diplotype . | Band 3/Spectrin . | Band 3/Protein 4.1 . | H2DIDS Binding Sites (μmol/L) . | Sulfate Flux . |
|---|---|---|---|---|---|
| . | . | . | . | . | (nmol/108 RBC/10 min) . |
| II.1 | Normal/Mondego | 94*-98* (−6) | 84-91 (−12) | 84 (−19) | 87 (−12.5) |
| III.3 | Normal/Mondego | 90-97* | 84-93* | 78 | 88 |
| I.2 | Normal/Coimbra | 81-85 (−18) | 73-76 (−27) | — (−35) | — (−34) |
| II.2 | Normal/Coimbra | 79-84 | 71-73 | 65 | 66 |
| III.1 | Coimbra/Mondego | 58-59 (−40) | 56-59 (−41) | 53 (−48) | 40 (−61.5) |
| III.2 | Coimbra/Mondego | 61-64 | 58-62 | 51 | 37 |
| Subject No. . | Diplotype . | Band 3/Spectrin . | Band 3/Protein 4.1 . | H2DIDS Binding Sites (μmol/L) . | Sulfate Flux . |
|---|---|---|---|---|---|
| . | . | . | . | . | (nmol/108 RBC/10 min) . |
| II.1 | Normal/Mondego | 94*-98* (−6) | 84-91 (−12) | 84 (−19) | 87 (−12.5) |
| III.3 | Normal/Mondego | 90-97* | 84-93* | 78 | 88 |
| I.2 | Normal/Coimbra | 81-85 (−18) | 73-76 (−27) | — (−35) | — (−34) |
| II.2 | Normal/Coimbra | 79-84 | 71-73 | 65 | 66 |
| III.1 | Coimbra/Mondego | 58-59 (−40) | 56-59 (−41) | 53 (−48) | 40 (−61.5) |
| III.2 | Coimbra/Mondego | 61-64 | 58-62 | 51 | 37 |
Band 3 ratios (band 3/spectrin and band 3/protein 4.1) were calculated on 4 gels28; the 2 ratios correspond to the lowest and highest values obtained from the 4 gels. H2DIDS binding sites and sulfate flux were obtained following extrapolation of experimental curves with the X and Y axes, respectively (not shown). Values in parentheses are the mean values of the reduction as a percentage of normal values.
Nonsignificant decrease (values considered individually) compared with the control (P < .05). All values without this symbol were significantly decreased.