Total PTP Activity in Normal and PV Erythroid Progenitor Cells
| Sample No. . | Normal . | PV . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | Age (yr) . | Sex . | Erythroid Colony Efficiency (%) . | Activity (U/mg) . | Age (yr) . | Sex . | Erythroid Colony Efficiency (%) . | Activity (U/mg) . |
| 1 | 48 | M | 57 | 0.22 | 67 | M | 59 | 0.34 |
| 2 | 28 | M | 57 | 0.12 | 69 | M | 67 | 0.25 |
| 3 | 52 | M | 63 | 0.09 | 38 | M | 70 | 0.32 |
| 4 | 35 | M | 75 | 0.07 | 49 | M | 57 | 0.27 |
| 5 | 28 | M | 65 | 0.13 | 70 | M | 54 | 0.28 |
| Mean ± SD | 38 ± 11 | 63 ± 7.9 | 0.13 ± 0.06 | 58 ± 14 | 61 ± 6.8 | 0.28 ± 0.05* | ||
| Sample No. . | Normal . | PV . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | Age (yr) . | Sex . | Erythroid Colony Efficiency (%) . | Activity (U/mg) . | Age (yr) . | Sex . | Erythroid Colony Efficiency (%) . | Activity (U/mg) . |
| 1 | 48 | M | 57 | 0.22 | 67 | M | 59 | 0.34 |
| 2 | 28 | M | 57 | 0.12 | 69 | M | 67 | 0.25 |
| 3 | 52 | M | 63 | 0.09 | 38 | M | 70 | 0.32 |
| 4 | 35 | M | 75 | 0.07 | 49 | M | 57 | 0.27 |
| 5 | 28 | M | 65 | 0.13 | 70 | M | 54 | 0.28 |
| Mean ± SD | 38 ± 11 | 63 ± 7.9 | 0.13 ± 0.06 | 58 ± 14 | 61 ± 6.8 | 0.28 ± 0.05* | ||
Normal and PV day-6 ECFC were collected and lysed in buffer A with 1% Triton X-100. PTP activity was assayed with 32P-labeled ENDYINASL as a substrate and presented as units per milligram of protein. Erythroid colony efficiency represents the percentage of erythroid colonies formed per cell number plated in the plasma clot cultures.
Significantly different from that of normal control group (P < .001, Student's t-test).