Table 1. Phenotype of CD34-Enriched Cell... | American Society of Hematology

Table 1.

Phenotype of CD34-Enriched Cell Fractions

Cell Surface	Percent Cells in Fraction
	Control CB^*				Experiment^†
	1	2	3	4	1	2	3	4	5^‡	6	7	8^‡
CD34⁺	95	92	89	75	85	92	95	78	100	85	99	100
CD33⁺	1	1.1	0.2	2.0	ND	ND	ND	ND	ND	ND	ND	ND
CD13⁺	0	0	0	0	ND	ND	ND	ND	ND	ND	ND	ND
CD14⁺	0	1	0.6	1.2	ND	ND	ND	ND	ND	ND	ND	ND
CD19⁺	1.2	2.3	1.8	6.2	ND	ND	ND	1.9	0	ND	6.4	0
CD2⁺	ND	2	5.2	13.9	ND	ND	ND	1.2	0	ND	0.6	ND
CD3⁺	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	0
glyc A⁺ CD71⁺ CD45⁻	0.6	0.15	2.9	3.8	ND	ND	ND	ND	ND	ND	ND	ND

Cell Surface	Percent Cells in Fraction
	Control CB^*				Experiment^†
	1	2	3	4	1	2	3	4	5^‡	6	7	8^‡
CD34⁺	95	92	89	75	85	92	95	78	100	85	99	100
CD33⁺	1	1.1	0.2	2.0	ND	ND	ND	ND	ND	ND	ND	ND
CD13⁺	0	0	0	0	ND	ND	ND	ND	ND	ND	ND	ND
CD14⁺	0	1	0.6	1.2	ND	ND	ND	ND	ND	ND	ND	ND
CD19⁺	1.2	2.3	1.8	6.2	ND	ND	ND	1.9	0	ND	6.4	0
CD2⁺	ND	2	5.2	13.9	ND	ND	ND	1.2	0	ND	0.6	ND
CD3⁺	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	0
glyc A⁺ CD71⁺ CD45⁻	0.6	0.15	2.9	3.8	ND	ND	ND	ND	ND	ND	ND	ND

Abbreviation: ND, not determined.

Cell populations in CB fractionated by double immunomagnetic selection.

Cell populations in fractionated CB used to engraft mice.

Cells were fractionated by one round of immunomagnetic selection followed by FACS for the CD34⁺ CD19⁻/2⁻(3⁻) fraction.