Binding Properties of mOSM and mLIF to Various Murine Cell Lines
| Cell Line . | 125I-ligand . | kd1* . | Sites/Cell . | kd2* . | Sites/Cell . |
|---|---|---|---|---|---|
| NIH3T3 | mOSM | 660 pmol/L | 6,710 | 1.52 nmol/L | 11,200 |
| mLIF | ND | ND | ND | ND | |
| M1 | mOSM | ND | ND | 4.25 nmol/L | 3,650 |
| mLIF | 139 pmol/L | 936 | ND | ND | |
| Ba/F3 | mOSM | ND | ND | ND | ND |
| mLIF | ND | ND | ND | ND | |
| Ba/F3-gp130 | mOSM | ND | ND | 2.79 nmol/L | 1,130 |
| mLIF | ND | ND | ND | ND | |
| Ba/F3-gp130 +LIFRĪ² | mOSM | ND | ND | 3.21 nmol/L | 448 |
| mLIF | 80.0 pmol/L | 443 | 1.87 nmol/L | 1,940 |
| Cell Line . | 125I-ligand . | kd1* . | Sites/Cell . | kd2* . | Sites/Cell . |
|---|---|---|---|---|---|
| NIH3T3 | mOSM | 660 pmol/L | 6,710 | 1.52 nmol/L | 11,200 |
| mLIF | ND | ND | ND | ND | |
| M1 | mOSM | ND | ND | 4.25 nmol/L | 3,650 |
| mLIF | 139 pmol/L | 936 | ND | ND | |
| Ba/F3 | mOSM | ND | ND | ND | ND |
| mLIF | ND | ND | ND | ND | |
| Ba/F3-gp130 | mOSM | ND | ND | 2.79 nmol/L | 1,130 |
| mLIF | ND | ND | ND | ND | |
| Ba/F3-gp130 +LIFRĪ² | mOSM | ND | ND | 3.21 nmol/L | 448 |
| mLIF | 80.0 pmol/L | 443 | 1.87 nmol/L | 1,940 |
Abbreviation: ND, not detected.
kd1 and kd2 are dissociation constants for high-affinity binding sites and low-affinity binding sites, respectively.