Phenotypic Analysis of B Lymphocytes From BM of C/EBPβ+/+ and C/EBPβ −/− Mice
. | Mice* . | |
---|---|---|
. | C/EBPβ+/+ . | C/EBPβ−/− . |
Total cell yield | 49.2 × 106 | 41.1 × 106 |
(4.9) | (6.8) | |
B220+ cells† | 14.2 | 6.1 |
(1.6) | (0.7) | |
B220+/IgM−† | 8.8 | 3.9 |
(1.0) | (0.4) | |
B220+ cell yield‡ | 7.0 × 106 | 2.4 × 106 |
(0.9) | (0.3) |
. | Mice* . | |
---|---|---|
. | C/EBPβ+/+ . | C/EBPβ−/− . |
Total cell yield | 49.2 × 106 | 41.1 × 106 |
(4.9) | (6.8) | |
B220+ cells† | 14.2 | 6.1 |
(1.6) | (0.7) | |
B220+/IgM−† | 8.8 | 3.9 |
(1.0) | (0.4) | |
B220+ cell yield‡ | 7.0 × 106 | 2.4 × 106 |
(0.9) | (0.3) |
The data are the mean of 26 C/EBPβ null mice and of an equal number of wild-type littermates. The relative standard deviations are shown in parentheses.
Single cell suspensions of cells were prepared from BM and analyzed by double fluorescence as detailed in Materials and Methods.
Values are expressed as percentages of B220+ or B220+/IgM− cells detected in the total BM cell population.
B220+ cell yield was calculated according to the percentages of B220+ BM cells.