Levels of Bioactive IL-7 in Culture Supernatants of BM Adherent Cells From C/EBPβ(+/+) and C/EBPβ(−/−) Mice
| . | IL-7 Production (ng/mL)* . | |
|---|---|---|
| . | Mice† . | . |
| . | . | . |
| . | C/EBPβ+/+ . | C/EBPβ−/− . |
| Exp 1 | 4.5 | 1.1†‡ |
| Exp 2 | 4.2 | 1.8 |
| Exp 3 | 5.1 | 2.1 |
| Exp 4 | 3.8 | 1.2 |
| Exp 5 | 4.5 | 2.0 |
| Exp 6 | 4.0 | 1.3 |
| Meanρ | 4.417 | 1.583 |
| Standard deviation | 0.426 | 0.436 |
| Significance | 0.001 | |
| . | IL-7 Production (ng/mL)* . | |
|---|---|---|
| . | Mice† . | . |
| . | . | . |
| . | C/EBPβ+/+ . | C/EBPβ−/− . |
| Exp 1 | 4.5 | 1.1†‡ |
| Exp 2 | 4.2 | 1.8 |
| Exp 3 | 5.1 | 2.1 |
| Exp 4 | 3.8 | 1.2 |
| Exp 5 | 4.5 | 2.0 |
| Exp 6 | 4.0 | 1.3 |
| Meanρ | 4.417 | 1.583 |
| Standard deviation | 0.426 | 0.436 |
| Significance | 0.001 | |
Supernatants collected 24 hours postfeeding were tested for IL-7 bioactivity by using the CT-6 cells.21 In parallel experiments, linear dilutions of neutralizing MoAb to IL-7 were added to the cultures. The data were compared with a standard curve of recombinant IL-7 (0.01 to 100 ng/mL).
Adherent cells were isolated from MPA-treated BM of C/EBPβ null mice and of wild-type littermates as detailed in Materials and Methods.
The data are corrected according to the inhibition observed in the cultures supplemented with the anti–IL-7 MoAb, and represent the actual levels of IL-7 production.
ρ The analysis of the data was performed according to the paired Student's t-statistic by using a StatWorks program (Cricket Software Inc, Philadelphia, PA).