CAFC Frequencies of Sorted BM CD34+FLT3+ and CD34+FLT3− Cells
| Fraction . | Experiment 1 . | Experiment 2 . | Experiment 3 . | |||
|---|---|---|---|---|---|---|
| . | Frequency . | Recovery (%) . | Frequency . | Recovery (%) . | Frequency . | Recovery (%) . |
| CD34+ | 1/50 | 100 | 1/200 | 100 | 1/80 | 100 |
| CD34+FLT3− | 1/80 | 15.6 | 1/250 | 17.6 | 1/110 | 19.6 |
| CD34+FLT3+ | 1/40 | 81 | 1/110 | 113 | 1/105 | 45.6 |
| Fraction . | Experiment 1 . | Experiment 2 . | Experiment 3 . | |||
|---|---|---|---|---|---|---|
| . | Frequency . | Recovery (%) . | Frequency . | Recovery (%) . | Frequency . | Recovery (%) . |
| CD34+ | 1/50 | 100 | 1/200 | 100 | 1/80 | 100 |
| CD34+FLT3− | 1/80 | 15.6 | 1/250 | 17.6 | 1/110 | 19.6 |
| CD34+FLT3+ | 1/40 | 81 | 1/110 | 113 | 1/105 | 45.6 |
CAFC frequency of MACS-selected CD34+ BM cells stained with anti–CD34-FITC and anti–FLT3-PE and FACS-sorted according to the sort windows shown in Fig 5B (right). Cells were ACDU sorted into 96-well plates coated with stromal FBMD-1 cells42 as described in Materials and Methods. CAFC frequencies were determined by Poisson statistics and Probit analysis was performed for comparison of groups.43