Colony Numbers of Sorted BM CD34+FLT3+ and CD34+FLT3− Cells
| Fraction . | Colonies . | |||
|---|---|---|---|---|
| . | BFU-E . | CFU-GM . | CFU-GEMM . | CFC Recovery (%) . |
| CD34+ | 57.2 ± 8.9 | 60.3 ± 16.1 | 1.6 ± 1.6 | 100 |
| CD34+FLT3− | 109.3 ± 13.5 | 22.6 ± 3.2 | 1.3 ± 0.8 | 21-28 |
| CD34+FLT3+ | 15.0 ± 3.2 | 94.7 ± 9.5 | 4.1 ± 3.8 | 53-63 |
| Fraction . | Colonies . | |||
|---|---|---|---|---|
| . | BFU-E . | CFU-GM . | CFU-GEMM . | CFC Recovery (%) . |
| CD34+ | 57.2 ± 8.9 | 60.3 ± 16.1 | 1.6 ± 1.6 | 100 |
| CD34+FLT3− | 109.3 ± 13.5 | 22.6 ± 3.2 | 1.3 ± 0.8 | 21-28 |
| CD34+FLT3+ | 15.0 ± 3.2 | 94.7 ± 9.5 | 4.1 ± 3.8 | 53-63 |
Colony-forming capacity of MACS-selected CD34+ BM cells stained with anti–CD34-FITC and anti–FLT3-PE and FACS-sorted according to the sort windows shown in Fig 5B (right). Fractionated cells were plated in semisolid methylcellulose cultures as described in Materials and Methods. Cultures were scored for colony growth after 14 days of incubation at 37°C and 5% CO2. Results represent the mean colony numbers ± SD based on 2 × 103 plated cells from three independent experiments with duplicate determinations.