Table 3. Determination of the Cell... | American Society of Hematology

Table 3.

Determination of the Cell Number, CFC, LTC-IC, and the Ability to Engraft the BM of NOD/SCID Recipients by Human Lin⁻CD34⁺ CB Cells Cultured on Allogeneic BM Stroma

No. of Experiment	Source of Cells	In Culture				% of Engraftment^3-151
		Cells ×10⁵	CFC	LTC-IC		Injected Into Mice
		per Flask (%)^3-150	per Flask (%)^3-150	per 10⁵	per Flask (%)^3-150	Cells ×10⁵ per Mouse	CFC	LTC-IC
							per Mouse	per Mouse
1	Lin⁻CD34⁺ CB	6.3	149,310	231.9	1,461	0.5	11,850	116	3; 26
	1 wk in culture	7.4 (117%)	95,090 (64%)	128.1	948 (65%)	2.0	25,700	256	1
						1.0	12,850	128	0; 0
						0.5	6,425	64	0; 0; 0
2	Lin⁻CD34⁺ CB	13.0	327,600	1,900.1	24,701	3.5	88,200	6,650	29; 71
	1 wk in culture	15.6 (120%)	113,880 (35%)	1,367.6	21,344 (86%)	7.8	56,940	10,667	30; 47
						3.9	28,470	5,334	11; 23

No. of Experiment	Source of Cells	In Culture				% of Engraftment^3-151
		Cells ×10⁵	CFC	LTC-IC		Injected Into Mice
		per Flask (%)^3-150	per Flask (%)^3-150	per 10⁵	per Flask (%)^3-150	Cells ×10⁵ per Mouse	CFC	LTC-IC
							per Mouse	per Mouse
1	Lin⁻CD34⁺ CB	6.3	149,310	231.9	1,461	0.5	11,850	116	3; 26
	1 wk in culture	7.4 (117%)	95,090 (64%)	128.1	948 (65%)	2.0	25,700	256	1
						1.0	12,850	128	0; 0
						0.5	6,425	64	0; 0; 0
2	Lin⁻CD34⁺ CB	13.0	327,600	1,900.1	24,701	3.5	88,200	6,650	29; 71
	1 wk in culture	15.6 (120%)	113,880 (35%)	1,367.6	21,344 (86%)	7.8	56,940	10,667	30; 47
						3.9	28,470	5,334	11; 23

F3-150

The value in parentheses represents the percentage of input value in the fresh BM sample.

F3-151

Each value represents the human cell engraftment in the BM of each individual mouse.

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