Table 2.

Determination of the Cell Number, CFC, LTC-IC, and the Ability to Engraft the BM of NOD/SCID Recipients by Human CB Cells Cultured on Allogeneic Stroma

No. of ExperimentSource of CellsIn Culture% of Engraftment†
Cells ×106 per Flask (%)*CFC per Flask (%)*LTC-ICInjected Into Mice
per 106per Flask (%)*Cells ×106 per MouseCFC per MouseLTC-IC per Mouse
1 Fresh CB 5.9 19,470 237.6 1,402 16.0 52,800 3,802 36 
 1 wk in culture 1.83 (31%) 34,404 (176%) 883.7 1,617 (115%) 16.0 300,800 14,139 62 
      8.0 150,400 7,070 4 
2 Fresh CB 8.0 15,200 135.7 1,086 16.8 31,920 2,280 3; 11 
      8.4 15,960 1,140 2; 10 
 1 wk in culture 2.15 (27%) 26,768 (176%) 598.9 1,288 (119%) 16.8 208,538 10,062 0; 2 
      8.4 104,269 5,031 0 
3 Fresh CB 10.0 53,500 313.8 3,138 16.8 89,880 5,272 41; 46 
      8.4 44,940 2,636 24; 34 
 1 wk in culture 2.4 (24%) 31,529 (59%) 206.6 496 (16%) 14.6 189,435 3,016 33; 37 
 2 wk in culture 4.4 (44%) 16,133 (30%) 10.7 47 (7%) 16.0 58,400 170 13; 20; 25 
      7.7 28,105 82 0.1 
      3.7 13,505 39 0.3 
No. of ExperimentSource of CellsIn Culture% of Engraftment†
Cells ×106 per Flask (%)*CFC per Flask (%)*LTC-ICInjected Into Mice
per 106per Flask (%)*Cells ×106 per MouseCFC per MouseLTC-IC per Mouse
1 Fresh CB 5.9 19,470 237.6 1,402 16.0 52,800 3,802 36 
 1 wk in culture 1.83 (31%) 34,404 (176%) 883.7 1,617 (115%) 16.0 300,800 14,139 62 
      8.0 150,400 7,070 4 
2 Fresh CB 8.0 15,200 135.7 1,086 16.8 31,920 2,280 3; 11 
      8.4 15,960 1,140 2; 10 
 1 wk in culture 2.15 (27%) 26,768 (176%) 598.9 1,288 (119%) 16.8 208,538 10,062 0; 2 
      8.4 104,269 5,031 0 
3 Fresh CB 10.0 53,500 313.8 3,138 16.8 89,880 5,272 41; 46 
      8.4 44,940 2,636 24; 34 
 1 wk in culture 2.4 (24%) 31,529 (59%) 206.6 496 (16%) 14.6 189,435 3,016 33; 37 
 2 wk in culture 4.4 (44%) 16,133 (30%) 10.7 47 (7%) 16.0 58,400 170 13; 20; 25 
      7.7 28,105 82 0.1 
      3.7 13,505 39 0.3 
*

The value in parentheses represents the percentage of input value in the fresh BM sample.

†

Each value represents the human cell engraftment in the BM of each individual mouse.

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