Table 1. Determination of the Cell... | American Society of Hematology

Table 1.

Determination of the Cell Number, CFC, LTC-IC, and the Ability to Engraft the BM of NOD/SCID Recipients by Human BM Cells Cultured on Allogeneic Stroma

No. of Experiment	Source of Cells	In Culture				% of Engraftment^†
		Cells ×10⁶	CFC	LTC-IC		Injected Into Mice
		per Flask (%)^*	per Flask (%)^*	per 10⁶	per Flask (%)^*	Cells ×10⁶ per Mouse	CFC per Mouse	LTC-IC
								per Mouse
1	Fresh BM	10.0	44,600	83.9	839	20.0	89,200	1,678	1; 1
	1 wk in culture	3.7 (37%)	25,245 (57%)	87.5	327 (39%)	20.0	135,000	1,750	0; 0.1
2	Fresh BM	10.0	74,000	209.0	2,090	10.2	75,480	2,132	0; 1
	1 wk in culture	5.8 (58%)	29,870 (40%)	134.9	782 (37%)	20.0	103,000	2,698	0; 0.03
						10.0	51,500	1,349	0; 0; 0.1
3	Fresh BM	10.0	48,000	118.1	1,181	19.5	93,600	2,303	17; 18
						10.0	48,000	1,151	0; 5
	1 wk in culture	8.7 (87%)	22,837 (48%)	97.7	850 (72%)	20.0	52,500	1,954	0; 0; 0.1; 2; 4
						10.0	26,250	977	0; 0.4; 2
	3 wk in culture	0.98 (9.8%)	3,714 (8%)	51.2	50 (5%)	13.5	51,165	691	0

No. of Experiment	Source of Cells	In Culture				% of Engraftment^†
		Cells ×10⁶	CFC	LTC-IC		Injected Into Mice
		per Flask (%)^*	per Flask (%)^*	per 10⁶	per Flask (%)^*	Cells ×10⁶ per Mouse	CFC per Mouse	LTC-IC
								per Mouse
1	Fresh BM	10.0	44,600	83.9	839	20.0	89,200	1,678	1; 1
	1 wk in culture	3.7 (37%)	25,245 (57%)	87.5	327 (39%)	20.0	135,000	1,750	0; 0.1
2	Fresh BM	10.0	74,000	209.0	2,090	10.2	75,480	2,132	0; 1
	1 wk in culture	5.8 (58%)	29,870 (40%)	134.9	782 (37%)	20.0	103,000	2,698	0; 0.03
						10.0	51,500	1,349	0; 0; 0.1
3	Fresh BM	10.0	48,000	118.1	1,181	19.5	93,600	2,303	17; 18
						10.0	48,000	1,151	0; 5
	1 wk in culture	8.7 (87%)	22,837 (48%)	97.7	850 (72%)	20.0	52,500	1,954	0; 0; 0.1; 2; 4
						10.0	26,250	977	0; 0.4; 2
	3 wk in culture	0.98 (9.8%)	3,714 (8%)	51.2	50 (5%)	13.5	51,165	691	0

The value in parentheses represents the percentage of input value in the fresh BM sample.

†

Each value represents the human cell engraftment in the BM of each individual mouse.

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