Primer Sets for Amplification of Each Exon and Intron
| . | Sense . | Antisense . |
|---|---|---|
| Promoter and 1st exon | 5′-GATGGTCTGATTCCAAAGCC-3′ | 5′-TGTGTACAGCCCTGAGAGAC-3′ |
| 2nd exon | 5′-CGAGGATCAAAGGCTTGGCT-3′ | 5′-GGCCAGTATAACTTGGAACT-3′ |
| 3rd exon | 5′-GGGGTACATTAGATATCAGC-3′ | 5′-ATTAGCCTAGCTCAGGACTG-3′ |
| 4th exon | 5′-CACATGCTTTCTTTTTCCCC-3′ | 5′-GATGCCTTCCCTATTCCGGT-3′ |
| 5th exon | 5′-ATACAGACTAGCCAGGGAGA-3′ | 5′-AAGTTTCCACTGCCAGCTCT-3′ |
| 6th exon | 5′-AACATTGACTCCCATCAAGG-3′ | 5′-CTGGATGCTGTATTGCAGGA-3′ |
| 7th exon | 5′-CCAGGTCAAACCTGAAGGTT-3′ | 5′-TTTTGTGAGACCAACACTAG-3′ |
| 8th exon | 5′-GAAGGTTATGATGTCCTACC-3′ | 5′-AAGAATTTTGTAAGGGCCTC-3′ |
| 9th exon | 5′-CATGATCCTGTTGCTCTGGA-3′ | 5′-AGCGTGAGGCTCCCAGAATA-3′ |
| 5′ of 9th exon | 5′-CATGATCCTGTTGCTCTGGA-3′ | 5′-GCATGTGCTTGACATTGCGC-3′ |
| 3′ of 9th exon | 5′-ACCCGTGACTTGGTGGACAT-3′ | 5′-AGCGTGAGGCTCCCAGAATA-3′ |
| 10th exon | 5′-ATCTGCTTAATGGAGCTAGT-3′ | 5′-AAACTCAGTGGTCTCTGTCC-3′ |
| nested | 5′-CCAGAGTGGCAGGTACTGTT-3′ | 5′-GCTTCTCTTTCAGATCCTGG-3′ |
| 11th exon | 5′-TGGCTCATCTGTACTGTGAC-3′ | 5′-TGACCAACAAGTGACCTATG-3′ |
| 3rd intron | 5′-GGGAAGAGCAAGATTGTGCA-3′ | 5′-GAATCAGGTGTGTGACCTTC-3′ |
| 4th intron | 5′-GAAGGTCACACACCTGATTC-3′ | 5′-ACACACGGTAGGTGTGATCC-3′ |
| . | Sense . | Antisense . |
|---|---|---|
| Promoter and 1st exon | 5′-GATGGTCTGATTCCAAAGCC-3′ | 5′-TGTGTACAGCCCTGAGAGAC-3′ |
| 2nd exon | 5′-CGAGGATCAAAGGCTTGGCT-3′ | 5′-GGCCAGTATAACTTGGAACT-3′ |
| 3rd exon | 5′-GGGGTACATTAGATATCAGC-3′ | 5′-ATTAGCCTAGCTCAGGACTG-3′ |
| 4th exon | 5′-CACATGCTTTCTTTTTCCCC-3′ | 5′-GATGCCTTCCCTATTCCGGT-3′ |
| 5th exon | 5′-ATACAGACTAGCCAGGGAGA-3′ | 5′-AAGTTTCCACTGCCAGCTCT-3′ |
| 6th exon | 5′-AACATTGACTCCCATCAAGG-3′ | 5′-CTGGATGCTGTATTGCAGGA-3′ |
| 7th exon | 5′-CCAGGTCAAACCTGAAGGTT-3′ | 5′-TTTTGTGAGACCAACACTAG-3′ |
| 8th exon | 5′-GAAGGTTATGATGTCCTACC-3′ | 5′-AAGAATTTTGTAAGGGCCTC-3′ |
| 9th exon | 5′-CATGATCCTGTTGCTCTGGA-3′ | 5′-AGCGTGAGGCTCCCAGAATA-3′ |
| 5′ of 9th exon | 5′-CATGATCCTGTTGCTCTGGA-3′ | 5′-GCATGTGCTTGACATTGCGC-3′ |
| 3′ of 9th exon | 5′-ACCCGTGACTTGGTGGACAT-3′ | 5′-AGCGTGAGGCTCCCAGAATA-3′ |
| 10th exon | 5′-ATCTGCTTAATGGAGCTAGT-3′ | 5′-AAACTCAGTGGTCTCTGTCC-3′ |
| nested | 5′-CCAGAGTGGCAGGTACTGTT-3′ | 5′-GCTTCTCTTTCAGATCCTGG-3′ |
| 11th exon | 5′-TGGCTCATCTGTACTGTGAC-3′ | 5′-TGACCAACAAGTGACCTATG-3′ |
| 3rd intron | 5′-GGGAAGAGCAAGATTGTGCA-3′ | 5′-GAATCAGGTGTGTGACCTTC-3′ |
| 4th intron | 5′-GAAGGTCACACACCTGATTC-3′ | 5′-ACACACGGTAGGTGTGATCC-3′ |